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RecombinantRabMAb

Recombinant Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

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Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunoprecipitation - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
  • Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3113] to Neuropilin 1 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
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Recombinant human Neuropilin 1 protein (Fc Chimera Active) (ab221023)

View more associated products

Overview

  • Product name

    Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free
    See all Neuropilin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3113] to Neuropilin 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Mouse
    See all applications and species data
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: HUVEC and HepG2 whole cell lysate (ab7900), human placenta, kidney and heart, mouse heart and kidney and rat heart and kidney tissue lysates. IHC-P: Human liver tissue; Rat brain tissue; Mouse brain tissue. ICC: MCF7 and HUVEC cells; Omentum and effluent-derived mesothelial cells; COS1 fibroblast-like cell line derived from monkey kidney tissue. Flow Cyt: HepG2 and MCF7 cells. IHC-Fr: Human kidney tissue. IP: Mouse heart tissue lysate
  • General notes

    ab184783 is the carrier-free version of ab81321. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab184783 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3113
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Neuropilin 1 antibody [EPR3113] (ab197644)
    • Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
    • PE Anti-Neuropilin 1 antibody [EPR3113] (ab209445)
    • Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Neuropilin 1 protein (Fc Chimera Active) (ab221023)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
IP
Mouse
All applications
Monkey
Common marmoset
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.

Can be blocked with Neuropilin 1 peptide (ab189308).

IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.

Can be blocked with Neuropilin 1 peptide (ab189308).

IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.

Target

  • Function

    The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
    The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
  • Tissue specificity

    The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
  • Sequence similarities

    Belongs to the neuropilin family.
    Contains 2 CUB domains.
    Contains 2 F5/8 type C domains.
    Contains 1 MAM domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession O14786 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8829 Human
    • Entrez Gene: 18186 Mouse
    • Entrez Gene: 246331 Rat
    • Omim: 602069 Human
    • SwissProt: O14786 Human
    • SwissProt: P97333 Mouse
    • SwissProt: Q9QWJ9 Rat
    • Unigene: 131704 Human
    • Unigene: 653996 Human
    • Unigene: 271745 Mouse
    • Unigene: 10815 Rat
    see all
  • Alternative names

    • A5 protein antibody
    • BDCA4 antibody
    • BLOOD DENDRITIC CELL ANTIGEN 4 antibody
    • CD304 antibody
    • Neuropilin-1 antibody
    • Neuropilin1 antibody
    • NP1 antibody
    • NPN1 antibody
    • NRP 1 antibody
    • NRP antibody
    • NRP1 antibody
    • NRP1_HUMAN antibody
    • transmembrane receptor antibody
    • Vascular endothelial cell growth factor 165 receptor antibody
    • VEGF165R antibody
    see all

Images

  • Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

    Flow Cytometry analysis of MCF7 cells labelling Neuropilin 1 with purified ab81321 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81321).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)Image from P?rez-Lozano ML et al. PLoS One. 2013;8(4):e60776. Fig 6.; doi: 10.1371/journal.pone.0060776.

    The expression of Neuropilin 1 and the mesothelial marker cytokeratin was analyzed in human peritoneal specimens by immunohistochemistry. Positive cells for antibodies used (Neuropilin 1 (ab81321) and Cytokeratin) show brown staining. Nuclei are counterstained in blue. (a, b) Control peritoneal tissue, with a conserved mesothelial cell monolayer showing an epithelioid morphology (with a 20X objective). These cells show weak expression of Neuropilin 1 and a marked staining for cytokeratin (arrows). No expression of these proteins was observed in the submesothelial area (region under mesothelial monolayer) (c, d) Fibrotic tissue sample from peritoneal dialysis (PD) patient showing the loss of mesothelial monolayer and invading spindle-like mesothelial cells in submesothelial area (with a 40X objective). These cells present a strong staining for Neuropilin 1 (c), and are also positive for cytokeratin (d) (arrows). Pictures are representative of 5 cases of PD patient samples and 4 of control samples.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81321).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)Image from P?rez-Lozano ML et al. PLoS One. 2013;8(4):e60776. Fig 5.; doi: 10.1371/journal.pone.0060776.

    The expression of Neuropilin 1, VEGFR-2, and VEGF was analyzed by immunofluorescence microscopy in omentum and effluent-derived mesothelial cells (MCs). MCs were double stained for Neuropilin 1 (green) and VEGFR-2 (red), and single stained for VEGF (green). Nuclei were stained with DAPI. Neuropilin 1 and VEGF show a membrane distribution in omentum and epithelioid MCs (b, c, h, i). During in vitro (e, f) and ex vivo (k, l) MMT both proteins change their localization and are internalized. The expression of VEGFR-2 is down-regulated but it does not show differences in localization during in vitro (a, d) and ex vivo (g, j) MMT.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81321).

  • Immunoprecipitation - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunoprecipitation - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

    Neuropilin 1 was immunoprecipitated from 0.35mg mouse heart lysate with ab81321 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab81321 at 1/1000 dilution (0.77 μg/mL). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.

    Lane 1: Mouse heart tissue lysate 10 μg
    Lane 2: Mouse heart tissue lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab81321 in mouse heart lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81321).

  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

    Clone EPR3113 (ab184783) has been successfully conjugated by Abcam. This image was generated using Anti-Neuropilin 1 antibody [EPR3113] (PE). Please refer to ab209445 for protocol details.

    ab209445 staining Neuropilin 1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209445 at 1/100 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

    Overlay histogram showing HepG2 cells stained with unpurified ab81321 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab81321, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81321).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Neuropilin 1 with purified ab81321 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81321).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)This image is courtesy of an Abreview submitted by Manoj Kumar Valluru.

    Unpurified ab81321 staining Neuropilin 1 in mouse brain tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in PBS + 2% blocking serum) for 16 hours at 4°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81321).

  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

    Clone EPR3113 (ab184783) has been successfully conjugated by Abcam. This image was generated using Anti-Neuropilin 1 antibody [EPR3113] (Alexa Fluor® 647). Please refer to ab198323 for protocol details.

    ab198323 staining Neuropilin 1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab198323 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

    Clone EPR3113 (ab184783) has been successfully conjugated by Abcam. This image was generated using Anti-Neuropilin 1 antibody [EPR3113] (Alexa Fluor® 488). Please refer to ab197644 for protocol details.

    ab197644 staining Neuropilin 1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197644 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

    Immunocytochemistry/Immunofluorescence analysis of HUVEC cells labelling Neuropilin 1 with purified ab81321 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81321).

  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)This image is courtesy of an anonymous Abreview.

    Unpurified ab81321 staining Neuropilin 1 in the COS1 fibroblast-like cell line derived from monkey kidney tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit monoclonal(1/500) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81321).

  • Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab184783? Please let us know so that we can cite the reference in this datasheet.

    ab184783 has not yet been referenced specifically in any publications.

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