Product nameAnti-Neuropilin 1 antibody [M534]
See all Neuropilin 1 primary antibodies
DescriptionMouse monoclonal [M534] to Neuropilin 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Neuropilin 1. Coupled to carrier protein. Corresponding to amino acids within the a1 CUB domain of human Neuropilin 1.
Database link: O14786
- Human recombinant Neuropilin 1; PC3 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab215743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).|
FunctionThe membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Tissue specificityThe expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
Sequence similaritiesBelongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- A5 protein antibody
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Lanes 1-2 : rabbit polyclonal anti-Neuropilin 1 at 1/250 dilution
Lanes 3-4 : Anti-Neuropilin 1 antibody [M534] (ab215743) at 1/250 dilution
Lanes 1 & 3 : Human recombinant Neuropilin 1
Lanes 2 & 4 : PC3 cell lysate
Predicted band size: 103 kDa
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, and 0.04% Tween20 for 1 h at room temperature.
ab215743 has not yet been referenced specifically in any publications.