Overview

  • Product name

    Anti-Neuropilin 1 antibody [M534]
    See all Neuropilin 1 primary antibodies
  • Description

    Mouse monoclonal [M534] to Neuropilin 1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Neuropilin 1. Coupled to carrier protein. Corresponding to amino acids within the a1 CUB domain of human Neuropilin 1.
    Database link: O14786

  • Positive control

    • Human recombinant Neuropilin 1; PC3 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab215743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).

Target

  • Function

    The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
    The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
  • Tissue specificity

    The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
  • Sequence similarities

    Belongs to the neuropilin family.
    Contains 2 CUB domains.
    Contains 2 F5/8 type C domains.
    Contains 1 MAM domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A5 protein antibody
    • BDCA4 antibody
    • BLOOD DENDRITIC CELL ANTIGEN 4 antibody
    • CD304 antibody
    • Neuropilin-1 antibody
    • Neuropilin1 antibody
    • NP1 antibody
    • NPN1 antibody
    • NRP 1 antibody
    • NRP antibody
    • NRP1 antibody
    • NRP1_HUMAN antibody
    • transmembrane receptor antibody
    • Vascular endothelial cell growth factor 165 receptor antibody
    • VEGF165R antibody
    see all

Images

  • Lanes 1-2 : rabbit polyclonal anti-Neuropilin 1 at 1/250 dilution
    Lanes 3-4 : Anti-Neuropilin 1 antibody [M534] (ab215743) at 1/250 dilution

    Lanes 1 & 3 : Human recombinant Neuropilin 1
    Lanes 2 & 4 : PC3 cell lysate

    Predicted band size: 103 kDa



    Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, and 0.04% Tween20 for 1 h at room temperature.

References

ab215743 has not yet been referenced specifically in any publications.

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