A comparison of leading NeuN antibodies in IHC
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We asked an independent academic collaborator to compare our NeuN RabMAb® antibody (ab177487) with the most cited mouse monoclonal via immunohistochemistry (IHC).
Results
- Our NeuN RabMAb antibody (ab177487) can be used at a lower dilution whilst still being as specific as the most cited mouse monoclonal.
- In contrast with the leading mouse monoclonal, ab177487 is compatible with both paraformaldehyde and acetone fixation.
- ab177487 was declared “excellent” for staining both frozen and paraffin-embedded sections.
Paraformaldehyde-fixed, paraffin-embedded adult mouse cerebellum sections
| Antibody | A. Leading mouse monoclonal | B. Anti-Neun-antibody [ERP12763] (ab177487) |
| Optimal dilution | 1/2500 | 1/5000 |
| Antigen retrieval | Sodium citrate | Sodium citrate |
| Staining pattern | Specific, with minimal staining of Purkinje neruons | Specific, with minimal staining of Purkinje neurons |
| Verdict | Excellent | Excellent |
Acetone-fixed adult mouse dentate gyrus frozen sections
| Antibody | A. Leading mouse monoclonal | B. Anti-NeuN antibody [EPR12763] (ab177487) |
| Optimal dilution | 1/2000 | 1/4000 |
| Staining pattern | Shows background that cannot be reduced by antibody dilution | Specific staining with minimal background |
| Verdict | Poor | Excellent |
Paraformaldehyde-fixed adult mouse sub ventricular zone frozen sections
| Antibody | A. Leading mouse monoclonal | B. Anti-NeuN antibody [EPR12763] (ab177487) |
| Optimal dilution | 1/2000 | 1/4000 |
| Staining pattern | Shows background that cannot be reduced by antibody dilution | Specific staining with minimal background |
| Verdict | Average | Excellent |
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