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Lewy bodies are intraneuronal aggregates of alpha-synuclein protein characteristic of Parkinson’s disease, Lewy body dementia, and other disorders. They are found in the regions of the brain responsible for motor control and result in a progressive decline in mental ability.
Figure 1. Alpha-synuclein fibril aggregate formation from the monomeric form.
The advantages of alpha-synuclein proteins
Assay alpha-synuclein proteins using thioflavin T (ThT)
Active alpha-synuclein aggregate seeds the formation of new alpha-synuclein aggregates from a pool of active alpha-synuclein monomers. ThT is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha-synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity.
Recombinant human alpha-synuclein protein monomer (active)
Recombinant human alpha-synuclein protein aggregate (active)
Recombinant human alpha-synuclein protein monomer (control)
Recombinant human alpha-synuclein protein aggregate (control)
Thioflavin T (ThT)
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