For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Alpha-synuclein protein assay protocol

Related

  • Browse all neuroinflammation resources
    • Active alpha-synuclein monomers and aggregates
      • Alpha-synuclein filament antibody
        • Alpha-synuclein SimpleStep ELISA (Human)

          Protocol for assaying active alpha-synuclein monomer and aggregate binding to thioflavin T.


          1. Prepare 1 mM stock solution of Thioflavin T (ThT) in dH2O (prepare fresh and filter through a 0.2 μm syringe filter).


          2. Dilute the ThT in PBS (pH 7.4) so that the final ThT concentration in each well is 25 μM.


          3. Thaw the alpha-synuclein (monomer and aggregate) aliquots on ice just before use.


          4. Either add 10 µM aggregate or 100 μM monomer (or both) to the appropriate wells.


          5. Seal and place the plate in a shaking incubator (600 rpm) at 37°C.


          6. Measure ThT fluorescence using a fluorescence microplate reader (excitation 450 nm, emission 485 nm) at 37°C from 1 hour up to 72 hours.


          ThT emission curves show increased fluorescence (correlated to alpha-synuclein protein aggregation) over time when 10 µM of active alpha-synuclein aggregate (ab218819) is combined with 100 µM of active alpha-synuclein monomer (ab218818) (light blue), as compared to when 100 µM of active alpha-synuclein monomer is combined with 10 µM of control alpha-synuclein aggregate (purple line), or 100 µM of control alpha-synuclein monomer (ab218816) is combined with 10 µM of control alpha-synuclein aggregate (ab218817) (dark blue). ThT ex = 450 nm, em = 485 nm.



          Figure 1. Graph showing ThT fluorescence following interactions with alpha-synuclein monomers, aggregates and controls.


          Products

          Product name

          Ab ID

          Recombinant human alpha-synuclein protein monomer (active)

          ab218818

          Recombinant human alpha-synuclein protein aggregate (active)

          ab218819

          Recombinant human alpha-synuclein protein monomer (control)

          ab218816

          Recombinant human alpha-synuclein protein aggregate (control)

          ab218817

          Thioflavin T (ThT)

          ab120751


          Get resources and offers direct to your inbox Sign up
          A-Z by research area
          • Cancer
          • Cardiovascular
          • Cell biology
          • Developmental biology
          • Epigenetics & Nuclear signaling
          • Immunology
          • Metabolism
          • Microbiology
          • Neuroscience
          • Signal transduction
          • Stem cells
          A-Z by product type
          • Primary antibodies
          • Secondary antibodies
          • Biochemicals
          • Isotype controls
          • Flow cytometry multi-color selector
          • Kits
          • Loading controls
          • Lysates
          • Peptides
          • Proteins
          • Slides
          • Tags and cell markers
          • Tools & Reagents
          Help & support
          • Support
          • Make an Inquiry
          • Protocols & troubleshooting
          • Placing an order
          • RabMAb products
          • Biochemical product FAQs
          • Training
          • Browse by Target
          Company
          • Corporate site
          • Investor relations
          • Company news
          • Careers
          • About us
          • Blog
          Events
          • Tradeshows
          • Conferences
          International websites
          • abcam.cn
          • abcam.co.jp

          Join with us

          • LinkedIn
          • facebook
          • Twitter
          • YouTube
          • Terms of sale
          • Website terms of use
          • Cookie policy
          • Privacy policy
          • Legal
          • Modern slavery statement
          © 1998-2022 Abcam plc. All rights reserved.