Immature neuron and intermediate progenitor markers
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A guide to choosing markers for immature neurons and intermediate progenitors.
We've pulled together the most common markers for immature neurons and intermediate progenitor cells, so you can quickly choose the right marker for your cell type.
Find your marker:
| Intermediate progenitor cells | Immature neurons |
| Doublecortin Beta III tubulin NeuroD1 TBR1 Stathmin 1 |
What are immature neurons and IPC?
Radial glia divides asymmetrically to produce one radial glial cell and one intermediate progenitor cell (IPC). IPCs differentiate into post-mitotic immature neurons, which migrate to their final destination in the nervous system and integrate into the neuronal network.
Intermediate progenitor cell (IPC) markers
TBR2
A transcription factor whose expression marks the transition from radial glia to intermediate progenitors.
Embryonic (E15) mouse cortical sections stained with anti-TBR2 (red) (ab23345).
MASH1 (Ascl1)
A transcription factor essential for neural differentiation. Please note that it can also label active neural stem cells.
Mouse subventricular zone tissue sections stained with anti-MASH1 (ab74065).
Immature neuron markers
Doublecortin
A microtubule-associated phosphoprotein that promotes neurite extension and cell migration.
Mouse adult dentate gyrus sections stained with anti-doublecortin (red) (ab18723).
Beta III tubulin
A class of tubulin whose expression is limited to neurons and is used to label immature neurons.
Mouse embryonic spinal cord stained with anti-beta III tubulin (red) (ab78078).
NeuroD1
A transcription factor that promotes neuronal development.
Mouse brain tissue stained with anti-neuroD1 (ab60704).
TBR1
A transcription factor whose expression marks the transition from intermediate progenitors to post-mitotic neurons.
Mouse brain sections stained with anti-TBR1 (red) (ab31940).
Stathmin 1
A cytoplasmic phosphoprotein involved in cytoskeletal regulation.
Mouse brain tissue sections stained with anti-stathmin 1 (ab109986).
References
- Atweh, C. The Role of Stathmin in the Regulation of the Cell Cycle. J.Cell.Biochem 93, (2004).
- Englund, C. et al. Pax6, Tbr2, and Tbr1 Are Expressed Sequentially by Radial Glia, Intermediate Progenitor Cells, and post-mitotic Neurons in Developing Neocortex. J. Neurosci. 25, 247–251 (2005).
- Katsetos, C. D. et al. Class III beta-tubulin isotype (beta III) in the adrenal medulla: I. Localization in the developing human adrenal medulla. Anat. Rec. 250, 335–43 (1998).
- Kim, E. J., Ables, J. L., Dickel, L. K., Eisch, A. J. & Johnson, J. E. Ascl1 (Mash1) Defines Cells with Long-Term Neurogenic Potential in Subgranular and Subventricular Zones in Adult Mouse Brain. (2011).
- Kwak, M. et al. Effects of Neonatal Hypoxic-Ischemic Injury and Hypothermic Neuroprotection on Neural Progenitor Cells in the Mouse Hippocampus. Dev. Neurosci. 37, 428–439 (2015).
- Martí-Mengual, U., Varea, E., Crespo, C., Blasco-Ibáñez, J. M. & Nacher, J. Cells expressing markers of immature neurons in the amygdala of adult humans. Eur. J. Neurosci. 37, 10–22 (2013).
- Menezes, J. R. & Luskin, M. B. Expression of neuron-specific tubulin defines a novel population in the proliferative layers of the developing telencephalon. J. Neurosci. 14, 5399–5416 (1994).
- Pollen, A. A. et al. Molecular Identity of Human Outer Radial Glia during Cortical Development. Cell 163, 55–67 (2015).
- Shin, J. et al. Single-Cell RNA-Seq with Waterfall Reveals Molecular Cascades underlying Adult Neurogenesis. Cell Stem Cell 17, 360–372 (2015).