NIRF probes for real-time imaging of amyloid beta
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Image amyloid beta (Aß) in real-time with near infrared fluorescent (NIRF) probes suitable for both in vitro and in vivo imaging.
The earliest pathological hallmark of Alzheimer’s disease is the deposition of Aß in the brain. Consequently, the past decade has seen the emergence of imaging probes for the non-invasive detection of Aß, mainly relying on techniques such as positron emission tomography (PET), single-photon emission computed tomography (SPECT) and magnetic resonance imaging (MRI). NIRF probes provide a safe, inexpensive, high resolution alternative method for real-time imaging of soluble and insoluble Aß in vivo and in vitro.
The advantages of NIRF probes
- Ideal for in vivo imaging
Emission wavelengths are in the NIR range, minimizing background fluorescence from brain tissue. - Detection of both soluble and insoluble Aß
We offer a comprehensive range of probes that allow the detection of both Aß monomers and fibrils (Figure 1). - Cell-permeable
All our NIRF probes can cross the blood brain barrier (BBB) allowing labeling of Aß in the brain. - Inexpensive
The imaging instrument costs are low, data analysis is quick, and highly skilled personnel are not required. - Safe to use
NIRF probes are non-radioactive, eliminating unnecessary exposure to radiation.
Available NIRF probes
| Product name | λex/λem (nm) | Aß species detected | References |
| MCAAD-3 (ab216983) | 596/685 | Aß42 fibrils | 1, 3 |
| CRANAD-2 (ab141775) | 640/715 | Aß40 fibrils | 1, 4 |
Figure 1. NIRF probes binding to Aß monomers and fibrils.
References
1. Xu, M.-M., Ren, W.-M., Tang, X.-C., Hu, Y.-H. & Zhang, H.-Y. Advances in development of fluorescent probes for detecting amyloid-β aggregates. Nat. Publ. Gr. 37, 719–730 (2016).
2. Chongzhao, R. et al. Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-ß deposits. J. Am. Chem. Soc. 131, 15257–15261 (2009).
3. Fu, H., Cui, M., Tu, P., Pan, Z. & Liu, B. Evaluation of molecules based on the electron donor-acceptor architecture as near-infrared β-amyloidal-targeting probes. Chem. Commun. (Camb). 50, 11875–8 (2014).
4. Chongzhao, R. et al. Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-ß deposits. J. Am. Chem. Soc. 131, 15257–15261 (2009).