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Hes5 antibody (ab25374)

Radial glia markers

Related

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                • Secondary antibodies
                  • Alexa Fluor® conjugated
                    • Anti-Rabbit IgG Alexa Fluor® 488 (ab150077)
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                      A guide to the most common radial glia markers.

                      We've put together the most commonly used markers for radial glia to help you quickly identify the suitable marker for your cells.

                      Find your marker:

                      Vimentin | PAX6 | HES1 and HES5 | Astrocytic markers: GFAP, GLAST, and BLBP | Adhesion and extracellular matrix molecules: TN-C and N-cadherin | Nestin | SOX2

                      What is radial glia?

                      During neurogenesis, neuroepithelial (NE) cells transform into radial glia. Epithelial features, such as tight junctions, are downregulated in favor of adherens junctions. Glial hallmarks, including astrocyte markers and morphological features such as glycogen granules, begin to emerge.

                      Radial glia markers

                      Vimentin

                      Vimentin is an intermediate filament protein, whose expression is upregulated during the epithelial-to-mesenchymal transition of NE cells to radial glia and persists until astrocyte development. 

                      We recommend Recombinant Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547). 

                      Recommended vimentin inhibitor and antiangiogenic agent: Withaferin A (ab120644)

                      Image (left): Rhesus monkey brain tissue sections stained with  anti-vimentin (ab92547).

                      Browse all vimentin antibodies

                      ​More recommended vimentin products and resources


                      PAX6

                      Paired box gene 6 or paired box protein (PAX6) is a transcription factor that promotes neurogenesis.

                      We recommend Anti-PAX6 antibody (ab5790). 

                      Image (left): Mouse embryonic (E12.5) brain sections stained with anti-PAX6 (ab5790).

                      Browse all PAX6 products


                      HES1 and HES5

                      These are transcription factors regulating the maintenance of radial glia.

                      We recommend Anti-HES5 antibody (ab25374). 

                      Image (left): Mouse brain tissue sections stained with anti-HES5 (red) (ab25374).

                      Browse all HES1 products

                      Browse all HES5 products


                      Astrocytic markers: GFAP, GLAST, and BLBP


                      These astrocytic markers emerge as neuroepithelial cells become radial glia.

                      We recommend Anti-BLBP antibody (ab32423). 

                      Recommended downregulator of GFAP expression: Methylprednisolone (ab142007)

                      Image (left): Rat embryonic (E16) spinal cord sections stained with anti-BLBP (ab32423).

                      Browse all GFAP products

                      Browse all GLAST products 

                      Browse all BLBP products


                      Adhesion and extracellular matrix molecules: TN-C and N-cadherin

                      Upregulation of adhesion and extracellular matrix proteins accompanies the transformation of NE cells into radial glia.

                      We recommend Recombinant Anti-N Cadherin antibody [EPR1791-4] (ab76011). 

                      Image (left): Mouse embryonic coronal cortical section stained with anti-N -cadherin (red) (ab76011).

                      Browse all TN-C products

                      Browse all N-cadherin products



                      Nestin

                      Nestin is an intermediate filament protein in NE cells and radial glia, whose expression persists until astrocyte development.

                      We recommend Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142). 

                      Image (left): Rat adult brain tissue sections stained with anti-nestin (ab6142).

                      Browse all nestin products


                      SOX2

                      SOX2 is a transcription factor and the earliest marker of the neural plate. It is expressed in proliferating cells and those acquiring glial fates but downregulated in post-mitotic neurons.

                      We recommend Anti-SOX2 antibody (ab97959). 

                      Image (left): Chicken embryonic (E7) brain tissue sections stained with anti-SOX2 (ab97959).

                      Browse all SOX2 products

                      References

                      • Bargagna-Mohan, P. et al. The tumor inhibitor and antiangiogenic agent withaferin A targets the intermediate filament protein vimentin. Chem. Biol. 14, 623–34 (2007).
                      • Bylund, M., Andersson, E., Novitch, B. G. & Muhr, J. Vertebrate neurogenesis is counteracted by Sox1-3 activity. Nat Neurosci 6, 1162–1168 (2003).
                      • Heins, N. et al. Glial cells generate neurons: the role of the transcription factor Pax6. Nat. Neurosci. 5, 308–315 (2002).
                      • Kageyama, R., Ohtsuka, T. & Kobayashi, T. Roles of Hes genes in neural development. Development Growth and Differentiation 50, (2008).
                      • Kriegstein, A. & Alvarez-Buylla, A. The glial nature of embryonic and adult neural stem cells. Annu. Rev. Neurosci. 32, 149–84 (2009).
                      • Kriegstein, A. R. & Götz, M. Radial glia diversity: A matter of cell fate. Glia 43, 37–43 (2003).
                      • Kriegstein, A. & Alvarez-Buylla, A. The glial nature of embryonic and adult neural stem cells. Annu. Rev. Neurosci. 32, 149–84 (2009).
                      • Lamouille, S., Xu, J. & Derynck, R. Molecular mechanisms of epithelial-mesenchymal transition. Nat. Rev. Mol. Cell Biol. 15, 178–96 (2014).
                      • Liu, W.-L. et al. Methylprednisolone inhibits the expression of glial fibrillary acidic protein and chondroitin sulfate proteoglycans in reactivated astrocytes. Glia 56, 1390–400 (2008).
                      • Papanayotou, C. et al. A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. Plos Biol 6, e2 (2008).


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