Key features and details
- Goat polyclonal to Neuroserpin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab32901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 48 kDa.
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSerine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
Tissue specificityPredominantly expressed in the brain.
Involvement in diseaseDefects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin.
Sequence similaritiesBelongs to the serpin family.
- Information by UniProt
- DKFZp781N13156 antibody
- Neuroserpin antibody
- NEUS_HUMAN antibody
Immunohistochemical analysis of paraffin embedded Human cortex tissue, staining Neuroserpin with ab32901 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Anti-Neuroserpin antibody (ab32901) at 1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer)
Predicted band size: 48 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab32901 has been referenced in 1 publication.
- Giampietro C et al. Cholesterol impairment contributes to neuroserpin aggregation. Sci Rep 7:43669 (2017). WB . PubMed: 28255164