Product nameAnti-Neurotensin Receptor 1/NTSR1 antibody
See all Neurotensin Receptor 1/NTSR1 primary antibodies
DescriptionRabbit polyclonal to Neurotensin Receptor 1/NTSR1
ab75555 detects endogenous levels of total Neurotensin receptor 1/NTSR1 protein.
Tested applicationsSuitable for: ICC/IF, WB, Indirect ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human Neurotensin Receptor 1/NTSR1 (internal sequence).
- Extracts from Jurkat cells
This product was previously labelled as Neurotensin Receptor 1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab75555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/1000. Predicted molecular weight: 46 kDa.|
RelevanceNeurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. NTR1 has been reported in brain, pancreas, and small intestine. ESTs have been isolated from brain and colon libraries.
Cellular localizationCell Membrane, Endoplasmic reticulum, Golgi Apparatus and Plasma membrane. Integral membrane protein.
- High affinity levocabastine insensitive neurotensin receptor antibody
- Neurotensin receptor 1 (high affinity) antibody
- Neurotensin receptor type 1 antibody
All lanes : Anti-Neurotensin Receptor 1/NTSR1 antibody (ab75555) at 1/500 dilution
Lane 1 : extracts from Jurkat cells
Lane 2 : extracts from Jurkat cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
ICC/IF image of ab75555 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75555, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab75555 has not yet been referenced specifically in any publications.