Overview

  • Product name

    Anti-Neurotrypsin antibody - Catalytic domain
  • Description

    Rabbit polyclonal to Neurotrypsin - Catalytic domain
  • Host species

    Rabbit
  • Specificity

    Recognizes the 875 and 834 amino acid forms of neurotrypsin. We have a range of domain specific antibodies for this target. For a full list please see all Neurotrypsin antibodies.
  • Tested applications

    Suitable for: IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide based on the catalytic domain of human Neurotrypsin.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 97 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.

Target

  • Function

    Plays a role in neuronal plasticity and the proteolytic action may subserve structural reorganizations associated with learning and memory operations.
  • Tissue specificity

    Brain and Leydig cells of the testis.
  • Involvement in disease

    Defects in PRSS12 are the cause of mental retardation autosomal recessive type 1 (MRT1) [MIM:249500]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
    Contains 4 SRCR domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • BSSP 3 antibody
    • BSSP3 antibody
    • Leydin antibody
    • MGC12722 antibody
    • Motopsin antibody
    • MRT1 antibody
    • NETR_HUMAN antibody
    • Neurotrypsin antibody
    • protease, serine, 12 (neurotrypsin, motopsin) antibody
    • Prss12 antibody
    • Serine protease 12 antibody
    see all

Images

  • All lanes : Anti-Neurotrypsin antibody - Catalytic domain (ab59454) at 1/1000 dilution

    Lane 1 : Tissue lysate prepared from rat testis
    at 15 µl
    Lane 2 : Tissue lysate prepared from rat testis
    at 10 µl
    Lane 3 : Tissue lysate prepared from mouse testis
    at 10 µl
    Lane 4 : Tissue lysate prepared from mouse testis
    at 8 µl
    Lane 5 : Tissue lysate prepared from mouse testis
    at 6 µl

    Predicted band size: 97 kDa

References

This product has been referenced in:

  • Spendiff S  et al. Denervation drives mitochondrial dysfunction in skeletal muscle of octogenarians. J Physiol 594:7361-7379 (2016). Read more (PubMed: 27619626) »
  • Meliopoulos VA  et al. MicroRNA regulation of human protease genes essential for influenza virus replication. PLoS One 7:e37169 (2012). WB ; Human . Read more (PubMed: 22606348) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (brain hemisphere coronal sections)
Specification
brain hemisphere coronal sections
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2 · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 15 2013

Question
Answer

Thank you very much for your interest our AbTrial program.

To our knowledge, ab59454 has not been tested in IHC-p or IHC-Fr. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.


The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Also more information about the RabMab promotion that I mentioned can be found at the following link, but please use the following code instead of the one mentioned in the link: RABMAB-XBSMG

https://www.abcam.com/index.html?pageconfig=resource&rid=15447



Please let me know if you have any questions about this offer and I would be happy to help you further.

Read More

Answer

According to our records, ab50454 was proving difficult to use inWestern blotand we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution. If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records. We wish you the best of luck with your research and look forward to a reply.

Read More

Answer

Thank you for contacting us and letting us know about the trouble with this antibody. I do have a couple of additional questions so that I can better understand the situation. Did you also probe for a loading control like beta actin? What is the difference between your control and experimental samples? Could you send an image? I looked through all of our information on this antibody and protein, and I don't have an explanation for why the band would be seen around 65 kDa. We do guarantee all of our products to work as stated on the datasheet for up to 6 months after purchase, so I would be happy to send a replacement or issue a credit or refund if you would like. I look forward to hearing from you. Please let me know if you have any additional questions or if there is anything else we can do for you.

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