The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 22 kDa.
Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
Involvement in disease
Defects in NRTN are a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
ab63949 staining Neurturin in Mouse brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in 4% PFA, followed by 30% sucrose and permeabilized with Triton X-100 prior to blocking with 10% goat serum for 20 minutes at 23°C. The primary antibody was diluted 1/200 in the blocking buffer and incubated with the sample for 90 minutes at 23°C. A biotin-conjugated Rabbit anti-Goat was used as the secondary antibody, diluted 1/200.