Neutral Red Assay Kit - Cell Viability / Cytotoxicity (ab234039)

Overview

  • Product name

    Neutral Red Assay Kit - Cell Viability / Cytotoxicity
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Product overview

    Neutral Red Assay Kit - Cell Viability/Cytotoxicity (ab234039) is simple, accurate and reproducible. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5,000-50,000 cells per well. It includes Doxorubicin as a positive control.


    Neutral red cell cytotoxicity assay is one of the common methods used to detect cell viability or drug cytotoxicity. The principle of this assay is based on the detection of viable cells via the uptake of the dye neutral red. Neutral red is a eurhodin dye that stains lysosomes in viable cells. Viable cells can take up neutral red via active transport and incorporate the dye into their lysosomes but non-viable cells cannot not take up this chromophore. Consequently, after washing, viable cells can release the incorporated dye in under acidifiedextracted conditions. The amount of released dye can be used to determine the total number of viable cells or drug cytotoxicity. The neutral red uptake assay provides a quantitative measurement of the number of viable cells and can be measured at OD 540 nm.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1000 tests
    100X Neutral Red Staining Solution 1 x 2ml
    10X Washing Solution 1 x 70ml
    20 mM Doxorubicin 1 x 100µl
    2X Solubilization Solution 1 x 75ml

Images

  • Dose-response curve of HepG2 (human liver hepatocellular carcinoma cell line) cells treated with doxorubicin for 72 hours determined using the Neutral Red Assay Kit - Cell Viability / Cytotoxicity (ab234039). Assays were performed according to the kit protocol in triplicate.

  • Dose-response curve of MCF7 (human breast adenocarcinoma cell line) cells treated with doxorubicin for 72 hours determined using the Neutral Red Assay Kit - Cell Viability / Cytotoxicity (ab234039). Assays were performed according to the kit protocol in triplicate.

Protocols

References

ab234039 has not yet been referenced specifically in any publications.

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