Overview

  • Product name
    Anti-Neutrophil antibody [7/4]
    See all Neutrophil primary antibodies
  • Description
    Rat monoclonal [7/4] to Neutrophil
  • Host species
    Rat
  • Specificity
    Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Neutrophil.

  • Epitope
    Recognizes the Ly-6B.2 antigen.
  • Positive control
    • Flow cytometry: Mouse peripheral blood granulocytes.
  • General notes

    Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab53457 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    7/4
  • Myeloma
    Y3/Ag1.2.3
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 25505239
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.

 

 

 

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Cellular localization
    Cell membrane; Lipid-anchor, GPI-anchor
  • Database links
  • Alternative names
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Images

  • Staining of mouse peripheral blood granulocytes with Rat anti mouse neutrophil (ab53457).

References

This product has been referenced in:
See all 15 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 12 2016

Question
Answer

Thank you for contacting us this afternoon and reporting the problems encountered with the most recent lot of the Anti-Neutrophil antibody [7/4] (ab53457). I am sorry to hear this antibody is not providing satisfactory results.

As discussed over the phone, it may be that you received a faulty vial. This is regrettable and I apologise for any inconvenience caused to you.I have arranged for a replacement vial of the antibody to be sent. This is on the order number 1154657. This should reach you tomorrow.

I have also attached a questionnaire so that I can collect a few more details of the problems encountered and the difference in staining observed between the different lots. This will greatly aid in investigating the problem further.

If you could also fill it out for the other antibody where you say you have been seeing some high background in the staining and I can have a look to see if there are any suggestions I can make to improve the staining.

As discussed over the phone, I have also issued a replacement of the Anti-F4/80 antibody [SP115] (ab111101). As this was purchased in August of last year, we would not normally offer to replace the antibody as this is well outside of the Abpromise warranty period. However, I have been willing to do this for you as a goodwill gesture. As agreed, if you could please submit an Abreview for the ab111101, detailing the antigen retrieval used and any advice that may be useful for future customers that would be very useful. As mentioned, detailed information of the experiment performed are not needed if you do not wish to share it before publishing in a journal. If you need help in this please do let me know.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Question

Dear Service-Team,

thank you for your answer. I would like to ask you for another antibody. Does

Anti-Neutrophil antibody [NIMP-R14] (ab2557) maybe work for VmDk mouse strain?



Thank you aganin for your help and best regards,



Hannah Schneider; Ph.D.


Postdoctoral Researcher
Department of Neurosurgery
Universitätsspital Zürich
Frauenklinikstrasse 10
CH-8091 Zürich

Tel. +41 44 63 48 940
Fax +41 44 63 48 951
Email: hannah.schneider@usz.ch




Von: technical@abcam.com [mailto:technical@abcam.com]
Gesendet: Montag, 16. Januar 2012 17:38
An: Schneider Hannah
Betreff: Reply from Abcam to your enquiry regarding ab53457 [CCE3427785]


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Dear service-team,



I have a question concerning the mouse anti-neutrophil antibody ab53457. Does this clone (7/4) also regognize neutrophils of the mouse strain Vm/Dk?



Thank you for your help.



Yours sincerely,



Hannah Schneider; Ph.D.



Postdoctoral Researcher

Department of Neurosurgery

Universitätsspital Zürich

Frauenklinikstrasse 10

CH-8091 Zürich



Tel. +41 44 63 48 940

Fax +41 44 63 48 951

Email: hannah.schneider@usz.ch












Best regards,
Keith

Keith Beadle
Scientific Support Specialist
Abcam Inc.
www.abcam.com
Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body
[CCE3427785]


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Read More
Answer

Thank you for contacting us.

This product has not been tested in that particular strain. However it is covered by our Abpromise and guaranteed to work on mouse in any of the tested applications listed on the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

Thank you for your inquiry. We have not tested MCA771GA on the Vm/Dk mouse strain. We ran a literature search, butwere unable to find any publications in which customers have tried this antibody on this mouse strain either.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying the one as per your requirements. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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