• Product name
    Anti-Neutrophil antibody [7/4] (FITC)
    See all Neutrophil primary antibodies
  • Description
    Rat monoclonal [7/4] to Neutrophil (FITC)
  • Host species
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: cultured bone marrow cells.

  • Epitope
    Recognizes the Ly-6B.2 antigen.
  • Positive control
    • Flow Cyt.: New Zealand Black mouse peripheral blood granulocytes.
  • General notes

    ab53453 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab53453 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Primary antibody notes
    ab53453 recognises a neutrophil allotypic marker, a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages.
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab53453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20038794
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Use 10µl of the working dilution to label 106 cells in 100µl.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.


  • Cellular localization
    Cell membrane; Lipid-anchor, GPI-anchor
  • Database links
  • Alternative names
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody


  • Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti mouse neutrophil:FITC (ab53453).
  • Immunohistochemical analysis of mouse spleen cryosection tissue stained for Neutrophil using ab53453.

  • Immunohistochemical analysis of acetone-fixed frozen mouse spleen, labelling Neutrophil with ab53453 at a dilution of 1/100 incubated for 1 hour at 25°C in 5% goat serum in TBS. The secondary used (to amplify the signal) was a Goat anti-rat polyclonal Alexa Fluor® 488 conjugate at a 1/400 dilution.

    See Abreview


This product has been referenced in:
  • Halim TYF  et al. Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid Cells. Immunity 48:1195-1207.e6 (2018). Read more (PubMed: 29907525) »
  • Florida SE  et al. In vivo structural and cellular remodeling of engineered bone-ligament-bone constructs used for anterior cruciate ligament reconstruction in sheep. Connect Tissue Res 57:526-538 (2016). Read more (PubMed: 27184487) »
See all 15 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A


Swiss PO is used to denote "Swiss-Pathology Oxford," a specific strain at Oxford University.

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Thank you for your enquiry. I am pleased to confirm the following information: ab105157 Datasheet suggests 10 ul per test 50 tests = 500 ul ab53453 On the datasheet, provide 50 ug at 0.1 mg/ml = 500ul I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying the one as per your requirements. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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