Flow Cytometry abreview for Anti-Neutrophil antibody [7/4] (FITC)

Poor
Abreviews
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive monocytes

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA 1%/Azide 0.01%

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
activated mouse macrophages are known to express 7/4. No binding detected in rat
Abcam response
Thank you for your valuable feedback.

We have not yet tested this antibody with rat cells, so it is possible that the antibody does not cross-react with this species.

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Submitted Apr 27 2012

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