Immunohistochemistry (Frozen sections) abreview for Anti-Neutrophil antibody [7/4] (FITC)

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen from immune mouse)
Permeabilization
No
Specification
Spleen from immune mouse
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% goat serum in TBS
Dilution
1/100

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: goat anti rat Alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
green: 7/4 in the red pulp zone secondary antibody has been used to enhance the signal

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Submitted Apr 18 2012

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