Anti-Neutrophil antibody [7/4] (Phycoerythrin) (ab105157)


  • Product name
    Anti-Neutrophil antibody [7/4] (Phycoerythrin)
    See all Neutrophil primary antibodies
  • Description
    Rat monoclonal [7/4] to Neutrophil (Phycoerythrin)
  • Host species
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Cultured Mouse bone marrow cells.

  • Epitope
    Recognizes the Ly-6B.2 antigen.
  • General notes

    ab105157 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab105157 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.



  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab105157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.


  • Cellular localization
    Cell membrane; Lipid-anchor, GPI-anchor
  • Database links
  • Alternative names
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody


  • Mouse (strain C57BL6) peripheral granulocytes stained with ab105157.


This product has been referenced in:
  • Cai Z  et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res N/A:N/A (2016). Read more (PubMed: 27444869) »
  • Rosas M  et al. The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J Leukoc Biol 88:169-80 (2010). Read more (PubMed: 20400676) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Q&A


You contacted Abcam back in November 2011 about a problem with ab105157 in flow cytometry. I was wondering if the problems had been resolved or if you are still having problems with it.

Please let me know if there is anything I can help you with.

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Thank you for your inquiry. The concentration of specific antibody in this product cannot be accurately calculated because of the interference in the reading from the PE protein itself. We can estimate that this product is 0.1mg/ml, but again, this is an estimate. I hope this information helps.  Please contact us with any other questions.    

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Thank you for your enquiry. I am pleased to confirm the following information: ab105157 Datasheet suggests 10 ul per test 50 tests = 500 ul ab53453 On the datasheet, provide 50 ug at 0.1 mg/ml = 500ul I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Thank you for contacting us and for including your attached flow data. I agree the vial of ab105157 you received is not working as expected. If you could forward me the purchase order number or Abcam order reference number associated with the purchase I would be happy to send you a replacement.

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Thank you for contacting Abcam. I am sorry that the current lot of ab105157 is not working in flow cytometry. The data you provide clearly shows that there is a difference in the results between the old and the new antibody. Would you be able to send me the lot number for the current antibody you have and the lot number for the old antibody, if you still have that information? I would like to see if there is any information about these specific lots that could account for such a difference. I look forward to your reply and helping you resolve this issue.

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Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying the one as per your requirements. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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