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    neutrophil-antibody-nimp-r14-ab2557.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Neutrophil antibody [NIMP-R14] (ab2557)

  • Datasheet
  • SDS
Reviews (23)Q&A (29)References (166)

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Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)

    Key features and details

    • Rat monoclonal [NIMP-R14] to Neutrophil
    • Suitable for: IHC-Fr
    • Reacts with: Mouse
    • Isotype: IgG2b

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    Overview

    • Product name

      Anti-Neutrophil antibody [NIMP-R14]
      See all Neutrophil primary antibodies
    • Description

      Rat monoclonal [NIMP-R14] to Neutrophil
    • Host species

      Rat
    • Tested applications

      Suitable for: IHC-Frmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils

    • Epitope

      The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.
    • General notes

      The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituent: 0.1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Primary antibody notes

      The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
    • Clonality

      Monoclonal
    • Clone number

      NIMP-R14
    • Myeloma

      210RCY3-Ag123
    • Isotype

      IgG2b
    • Light chain type

      unknown
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Cardiovascular
      • Blood
      • Other

    Associated products

    • Alternative Versions

      • PE Anti-Neutrophil antibody [NIMP-R14] (ab125159)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2557 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr (4)
    Use at an assay dependent concentration. Tested with acetone-fixed tissue.
    Notes
    IHC-Fr
    Use at an assay dependent concentration. Tested with acetone-fixed tissue.

    Target

    • Cellular localization

      Cell membrane; Lipid-anchor, GPI-anchor
    • Database links

      • Entrez Gene: 110453 Mouse
      • Alternative names

        • Ly-6B antibody
        • Ly6b antibody
        • Lymphocyte antigen 6B antibody
        • Lymphocyte antigen 6B Short name=Ly-6B antibody

      Images

      • Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)
        Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)

        Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (166)

      Publishing research using ab2557? Please let us know so that we can cite the reference in this datasheet.

      ab2557 has been referenced in 166 publications.

      • Qian J  et al. Upregulation of PDGF Mediates Robust Liver Regeneration after Nanosecond Pulsed Electric Field Ablation by Promoting the HGF/c-Met Pathway. Biomed Res Int 2020:3635787 (2020). PubMed: 32258116
      • Kim KW  et al. FUT1 deficiency elicits immune dysregulation and corneal opacity in steady state and under stress. Cell Death Dis 11:285 (2020). PubMed: 32332708
      • Ryu G  et al. Role of IL-17A in Chronic Rhinosinusitis With Nasal Polyp. Allergy Asthma Immunol Res 12:507-522 (2020). PubMed: 32141263
      • Park CJ  et al. Progesterone Receptor Serves the Ovary as a Trigger of Ovulation and a Terminator of Inflammation. Cell Rep 31:107496 (2020). PubMed: 32294429
      • Kong AM  et al. Bio-engineering a tissue flap utilizing a porous scaffold incorporating a human induced pluripotent stem cell-derived endothelial cell capillary network connected to a vascular pedicle. Acta Biomater 94:281-294 (2019). PubMed: 31152943
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 52 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Mouse Liver Tissue)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate, pH 6.0
      Permeabilization
      No
      Specification
      Mouse Liver Tissue
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 23 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Antigen retrieval step
      None
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 26 2020

      Immunohistochemistry (Frozen sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Lung)
      Permeabilization
      No
      Specification
      Lung
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Mr. Carl Hobbs

      Verified customer

      Submitted Jun 09 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (brain tissue)
      Antigen retrieval step
      None
      Permeabilization
      No
      Specification
      brain tissue
      Blocking step
      Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
      Fixative
      Formaldehyde
      Read More

      Herr Dr. Markus Kipp

      Verified customer

      Submitted Sep 07 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (spleen)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
      Permeabilization
      No
      Specification
      spleen
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
      Fixative
      10% NBF
      Read More

      Abcam user community

      Verified customer

      Submitted May 10 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (lung)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TE pH9
      Permeabilization
      No
      Specification
      lung
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 18 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
      Permeabilization
      No
      Specification
      Kidney
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Dr. Wesley Konsavage

      Verified customer

      Submitted Dec 22 2016

      Immunohistochemistry (Frozen sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Skin)
      Permeabilization
      No
      Specification
      Skin
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Dr. Victoria Bridgeman

      Verified customer

      Submitted Jun 23 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Skin)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ph6 citrate
      Permeabilization
      No
      Specification
      Skin
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Dr. Victoria Bridgeman

      Verified customer

      Submitted Jun 23 2016

      Flow Cytometry abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Fixation
      None, cells were used fresh
      Permeabilization
      No
      Sample
      Mouse Cell (Cells from bronchoaveolar lavage in balb/c mice)
      Specification
      Cells from bronchoaveolar lavage in balb/c mice
      Gating Strategy
      alive (DAPI-),CD45+, C11b+, CD11c- cells
      Preparation
      Cell harvesting/tissue preparation method: Bronchoaveolar lavage in balb/c mice was performed with PBS-EDTA 3mM
      Sample buffer: PBS-EDTA 3mM
      Read More

      Dr. Francois Daubeuf

      Verified customer

      Submitted Feb 20 2015

      1-10 of 52 Abreviews or Q&A

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