Anti-Neutrophil antibody [NIMP-R14] (ab2557)
![Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.com/ps/products/2/ab2557/Images/ab2557-282294-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)")
Key features and details
- Rat monoclonal [NIMP-R14] to Neutrophil
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2b
Overview
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Product name
Anti-Neutrophil antibody [NIMP-R14]
See all Neutrophil primary antibodies -
Description
Rat monoclonal [NIMP-R14] to Neutrophil -
Host species
Rat -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils
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Epitope
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. -
General notes
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. -
Clonality
Monoclonal -
Clone number
NIMP-R14 -
Myeloma
210RCY3-Ag123 -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (4) |
Use at an assay dependent concentration. Tested with acetone-fixed tissue.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. Tested with acetone-fixed tissue. |
Target
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Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor -
Database links
- Entrez Gene: 110453 Mouse
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Alternative names
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
Images
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Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)
Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.
Protocols
References (166)
ab2557 has been referenced in 166 publications.
- Qian J et al. Upregulation of PDGF Mediates Robust Liver Regeneration after Nanosecond Pulsed Electric Field Ablation by Promoting the HGF/c-Met Pathway. Biomed Res Int 2020:3635787 (2020). PubMed: 32258116
- Kim KW et al. FUT1 deficiency elicits immune dysregulation and corneal opacity in steady state and under stress. Cell Death Dis 11:285 (2020). PubMed: 32332708
- Ryu G et al. Role of IL-17A in Chronic Rhinosinusitis With Nasal Polyp. Allergy Asthma Immunol Res 12:507-522 (2020). PubMed: 32141263
- Park CJ et al. Progesterone Receptor Serves the Ovary as a Trigger of Ovulation and a Terminator of Inflammation. Cell Rep 31:107496 (2020). PubMed: 32294429
- Kong AM et al. Bio-engineering a tissue flap utilizing a porous scaffold incorporating a human induced pluripotent stem cell-derived endothelial cell capillary network connected to a vascular pedicle. Acta Biomater 94:281-294 (2019). PubMed: 31152943
