Anti-Neutrophil antibody [NIMP-R14] (ab2557)
![Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.com/ps/products/2/ab2557/Images/ab2557-282294-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)")
Key features and details
- Rat monoclonal [NIMP-R14] to Neutrophil
- Suitable for: ICC, IHC-Fr, IHC-P, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-Neutrophil antibody [NIMP-R14]
See all Neutrophil primary antibodies -
Description
Rat monoclonal [NIMP-R14] to Neutrophil -
Host species
Rat -
Tested applications
Suitable for: ICC, IHC-Fr, IHC-P, Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils
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Epitope
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. -
General notes
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. -
Clonality
Monoclonal -
Clone number
NIMP-R14 -
Myeloma
210RCY3-Ag123 -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | (3) |
Use at an assay dependent concentration.
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| IHC-Fr | (4) |
Use at an assay dependent concentration. Tested with acetone-fixed tissue.
|
| IHC-P | (15) |
Use at an assay dependent concentration.
This antibody has been reported to work well without antigen retrieval, or with retrieval in a 125 degree C decloaking chamber for 3 minutes with pH 6.5 buffer. HIER optimization may be required. |
| Flow Cyt | (1) |
Use at an assay dependent concentration.
5 x 10^5 cells were incubated with 10 ug/ml antibody. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Functional Studies |
Use at an assay dependent concentration.
Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1 mg, 6 hours before infection. |
| Notes |
|---|
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ICC
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. Tested with acetone-fixed tissue. |
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IHC-P
Use at an assay dependent concentration. This antibody has been reported to work well without antigen retrieval, or with retrieval in a 125 degree C decloaking chamber for 3 minutes with pH 6.5 buffer. HIER optimization may be required. |
|
Flow Cyt
Use at an assay dependent concentration. 5 x 10^5 cells were incubated with 10 ug/ml antibody. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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Functional Studies
Use at an assay dependent concentration. Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1 mg, 6 hours before infection. |
Target
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Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor -
Database links
- Entrez Gene: 110453 Mouse
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Alternative names
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
Images
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Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)
Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (200)
ab2557 has been referenced in 200 publications.
- Eliseeva SI et al. Changes in lung immune cell infiltrates after electric field treatment in mice. Sci Rep 11:1453 (2021). PubMed: 33446928
- Molenaar B et al. Single-cell transcriptomics following ischemic injury identifies a role for B2M in cardiac repair. Commun Biol 4:146 (2021). PubMed: 33514846
- Miao AF et al. Hypoxia-inducible factor prolyl hydroxylase inhibitor roxadustat (FG-4592) protects against renal ischemia/reperfusion injury by inhibiting inflammation. Ren Fail 43:803-810 (2021). PubMed: 33966598
- Brunner SM et al. Galanin receptor 3 attenuates inflammation and influences the gut microbiota in an experimental murine colitis model. Sci Rep 11:564 (2021). PubMed: 33436730
- Hazlewood JE et al. Injection site vaccinology of a recombinant vaccinia-based vector reveals diverse innate immune signatures. PLoS Pathog 17:e1009215 (2021). PubMed: 33439897