Anti-Neutrophil antibody [NIMP-R14] (ab2557)
Rat monoclonal Neutrophil antibody [NIMP-R14]. Validated in IHC, FuncS, ICC, Flow Cyt and tested in Mouse. Cited in 98 publication(s). Independently reviewed in 20 review(s).
- Datasheet
- References (130)
- Protocols
Overview
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Product name
Anti-Neutrophil antibody [NIMP-R14]
See all Neutrophil primary antibodies -
Description
Rat monoclonal [NIMP-R14] to Neutrophil -
Host species
Rat -
Tested applications
Suitable for: ICC, Functional Studies, IHC-P, Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils
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Epitope
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. -
General notes
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. -
Clonality
Monoclonal -
Clone number
NIMP-R14 -
Myeloma
210RCY3-Ag123 -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| Functional Studies | Use at an assay dependent concentration. Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1 mg, 6 hours before infection. | |
| IHC-P | Use at an assay dependent concentration. This antibody has been reported to work well without antigen retrieval, or with retrieval in a 125 degree C decloaking chamber for 3 minutes with pH 6.5 buffer. HIER optimization may be required. |
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| Flow Cyt | Use at an assay dependent concentration. 5 x 105 cells were incubated with 10 µg/ml antibody. |
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| IHC-Fr | Use at an assay dependent concentration. Tested with acetone-fixed tissue. |
Target
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Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor -
Database links
- Entrez Gene: 110453 Mouse
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Alternative names
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.com/ps/products/2/ab2557/Images/ab2557-320034-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)Mukai K et al. The effect of 17ß-estradiol on cutaneous wound healing in protein-malnourished ovariectomized female mouse model. PLoS One 9:e115564 (2014). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/4% paraformaldehyde-fixed mouse uterus tissue 3 or 7 days after wounding, stained for Neutrophil using ab2557 at 1/100 dilution in immunohistochemical analysis.
Slides from let to right include: Control SHAM, Protein-malnurished SHAM, protein-malnourished ovariectomized (OVX) female mice, and PM OVX mice treated with 17β-estradiol.
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![Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.com/ps/products/2/ab2557/Images/ab2557-282294-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.
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![Immunocytochemistry - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.com/ps/products/2/ab2557/Images/ab2557-5944-anti-neutrophil-antibody-nimp-r14-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Neutrophil antibody [NIMP-R14] (ab2557)ab2557 at 1/80 staining mouse blood cells (ip wash cells) by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a biotinylated rabbit anti-rat (mouse adsorbed) antibody.
This image is courtesy of an Abreview submitted on 16 March 2006.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.com/ps/products/2/ab2557/Images/ab2557-260762-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)This image is courtesy of an anonymous Abreviewab2557 staining Neutrophil in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM sodium citrate. Samples were incubated with primary antibody (1/200 in 1x PBS/1% BSA/0.3% Triton X-100 buffer) for 1 hour at 25°C. A HRP-conjugated Goat anti-rat Ig polyclonal (1/200) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.com/ps/products/2/ab2557/Images/ab2557-5943-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)Image courtesy of an anonymous Abreview.ab2557 staining Neutrophil in mouse tumor tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked, followed by incubation with the primary antibody at a 1/50 dilution for 30 minutes. A biotin-conjugated rabbit anti-rat was used as secondary antibody at a 1/400 dilution.
Protocols
References
This product has been referenced in:
- Germán B et al. Disrupting the IL-36 and IL-23/IL-17 loop underlies the efficacy of calcipotriol and corticosteroid therapy for psoriasis. JCI Insight 4:N/A (2019). Read more (PubMed: 30674716) »
- Ferguson B et al. Different genetic mechanisms mediate spontaneous versus UVR-induced malignant melanoma. Elife 8:N/A (2019). Read more (PubMed: 30681412) »
