Overview

  • Product name
    Anti-Neutrophil Bactericidal Protein BP30 antibody
    See all Neutrophil Bactericidal Protein BP30 primary antibodies
  • Description
    Rabbit polyclonal to Neutrophil Bactericidal Protein BP30
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Neutrophil Bactericidal Protein BP30 aa 293-324 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P17213

  • Positive control
    • IHC-P: Human tonsil and lung tissues. WB: HL-60 cell lysate. Flow Cyt: HL-60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.
IHC-P 1/50 - 1/100.
WB 1/1000. Predicted molecular weight: 54 kDa.

Target

  • Function
    The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
  • Tissue specificity
    Restricted to cells of the myeloid series.
  • Sequence similarities
    Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
  • Domain
    The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N-and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested.
  • Cellular localization
    Secreted. Cytoplasmic granule membrane. Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bactericidal permeability-increasing protein antibody
    • Bactericidal/permeability increasing protein antibody
    • BPI antibody
    • BPI fold containing family D, member 1 antibody
    • BPI_HUMAN antibody
    • BPIFD1 antibody
    • CAP 57 antibody
    • CAP57 antibody
    • rBPI antibody
    • Recombinant BPI holoprotein, rBPI antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human lung tissue stained for Neutrophil Bactericidal Protein BP30 using ab230452 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometry analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling Neutrophil Bactericidal Protein BP30 with ab230452 at 1/10 dilution (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-Neutrophil Bactericidal Protein BP30 antibody (ab230452) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate at 35 µg

    Predicted band size: 54 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Neutrophil Bactericidal Protein BP30 using ab230452 at 1/100 dilution in immunohistochemical analysis, followed by peroxidase-conjugated secondary antibody and DAB staining.

References

ab230452 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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