Neutrophil Elastase Activity Assay Kit (Fluorometric) (ab204730)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Neutrophil Elastase Activity Assay Kit (Fluorometric)
See all Neutrophil Elastase kits -
Detection method
Fluorescent -
Sample type
Plasma, Whole Blood, Purified protein -
Assay type
Enzyme activity -
Sensitivity
1 ng -
Assay time
0h 20m -
Species reactivity
Reacts with: Other species, Mammals -
Product overview
Neutrophil Elastase Activity Assay Kit (Fluorometric) ab204730 is based on the ability of neutrophil elastase (NE) to proteolytically cleave a synthetic substrate in order to release a fluorophore, AFC (Ex/Em 380/500 nm), which can be easily quantified by fluorescence microplate readers.
This assay kit is simple, rapid and can detect as low as 1 ng of Neutrophil Elastase in a variety of samples.
Neutrophil elastase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader every 2-3 min for 10-20 min -
Notes
Neutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil elaszym, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it causes degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests NE Assay Buffer 1 x 15ml NE Dilution Buffer 1 x 1ml NE Enzyme Standard 1 x 1µg NE Substrate 1 x 200µl -
Research areas
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Relevance
Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage. -
Alternative names
- Bone marrow serine protease
- ELA2
- ELANE
see all
Images
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Neutrophil Elastase Activity Assay Kit (Fluorometric) (ab204730)Typical Neutrophil Elastase standard curve.
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Neutrophil Elastase Activity Assay Kit (Fluorometric) (ab204730)Neutrophil Elastase activity was measured in human blood, following removal of RBCs and stimulation of neutrophils by 100 nM PMA. S = Substrate, I = Inhibitor (60 µM SPCK).
Protocols
References
This product has been referenced in:
- Sesma JI et al. SPX-101 is stable in and retains function after exposure to cystic fibrosis sputum. J Cyst Fibros N/A:N/A (2018). Read more (PubMed: 29936069) »
- Ajendra J et al. NOD2 dependent neutrophil recruitment is required for early protective immune responses against infectious Litomosoides sigmodontis L3 larvae. Sci Rep 6:39648 (2016). Read more (PubMed: 28004792) »
