Product nameNeutrophil Elastase Activity Assay Kit (Fluorometric)
See all Neutrophil Elastase kits
Sample typePlasma, Whole Blood, Purified protein
Assay typeEnzyme activity
Assay time0h 20m
Species reactivityReacts with: Other species, Mammals
Neutrophil Elastase Activity Assay Kit (Fluorometric) ab204730 is based on the ability of neutrophil elastase (NE) to proteolytically cleave a synthetic substrate in order to release a fluorophore, AFC (Ex/Em 380/500 nm), which can be easily quantified by fluorescence microplate readers.
This assay kit is simple, rapid and can detect as low as 1 ng of Neutrophil Elastase in a variety of samples.
Neutrophil elastase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader every 2-3 min for 10-20 min
Neutrophil Elastase (NE, EC 188.8.131.52, leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil elaszym, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it causes degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests NE Assay Buffer 1 x 15ml NE Dilution Buffer 1 x 1ml NE Enzyme Standard 1 x 1µg NE Substrate 1 x 200µl
RelevanceNeutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage.
- Bone marrow serine protease
ab204730 has been referenced in 3 publications.
- Sesma JI et al. SPX-101 is stable in and retains function after exposure to cystic fibrosis sputum. J Cyst Fibros N/A:N/A (2018). PubMed: 29936069
- Zhao H et al. A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise. Mol Cancer Ther 16:1866-1876 (2017). PubMed: 28522588
- Ajendra J et al. NOD2 dependent neutrophil recruitment is required for early protective immune responses against infectious Litomosoides sigmodontis L3 larvae. Sci Rep 6:39648 (2016). PubMed: 28004792