Overview

  • Product name

    Neutrophil Elastase Activity Assay Kit (Fluorometric)
    See all Neutrophil Elastase kits
  • Detection method

    Fluorescent
  • Sample type

    Plasma, Whole Blood, Purified protein
  • Assay type

    Enzyme activity
  • Sensitivity

    1 ng
  • Assay time

    0h 20m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Neutrophil Elastase Activity Assay Kit (Fluorometric) ab204730 is based on the ability of neutrophil elastase (NE) to proteolytically cleave a synthetic substrate in order to release a fluorophore, AFC (Ex/Em 380/500 nm), which can be easily quantified by fluorescence microplate readers.


    This assay kit is simple, rapid and can detect as low as 1 ng of Neutrophil Elastase in a variety of samples.


    Neutrophil elastase assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with a microplate reader every 2-3 min for 10-20 min

  • Notes

    Neutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil elaszym, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it causes degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    NE Assay Buffer 1 x 15ml
    NE Dilution Buffer 1 x 1ml
    NE Enzyme Standard 1 x 1µg
    NE Substrate 1 x 200µl
  • Research areas

  • Relevance

    Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage.
  • Alternative names

    • Bone marrow serine protease
    • ELA2
    • ELANE
    • Elastase 2
    • Elastase 2 neutrophil
    • Elastase neutrophil expressed
    • GE
    • Granulocyte derived elastase
    • HLE
    • HNE
    • Human leukocyte elastase
    • Leukocyte elastase
    • Medullasin
    • NE
    • PMN E
    • PMN elastase
    • Polymorphonuclear elastase
    • SCN1
    see all

Images

  • Typical Neutrophil Elastase standard curve.

  • Neutrophil Elastase activity was measured in human blood, following removal of RBCs and stimulation of neutrophils by 100 nM PMA. S = Substrate, I = Inhibitor (60 µM SPCK).

Protocols

References

This product has been referenced in:

  • Sesma JI  et al. SPX-101 is stable in and retains function after exposure to cystic fibrosis sputum. J Cyst Fibros N/A:N/A (2018). Read more (PubMed: 29936069) »
  • Ajendra J  et al. NOD2 dependent neutrophil recruitment is required for early protective immune responses against infectious Litomosoides sigmodontis L3 larvae. Sci Rep 6:39648 (2016). Read more (PubMed: 28004792) »
See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
We tested NE activity assay kit from mice plasma.
Methods and results are depicted in the additional file.
Best regards
Dr Jarrot

Mr. Pierre Jarrot

Verified customer

Submitted Mar 24 2017

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