Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
None - Buffer/Enzyme Used: 10mM sodium citrate, ph 6.0
Sample
Baboon Tissue sections (Neutrophils in Baboon placenta)
Specification
Neutrophils in Baboon placenta
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% normal goat serum in PBS
Dilution
1/250
Secondary antibody
Concentration
7 µg/ml
Name
Non-Abcam antibody was used: biotinylated goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Tried heat-mediated antigen retrieval with 10mM sodium citrate buffer, pH 6.0 but saw no signal. Used tertiary detection with Streptavidin-AlexaFluor 488 at 1:500 dilution for 30 min followed by quenching of autofluorescence with 1% Sudan Black in 70% methanol for 10 min, both at RT. Image: green=protein, red=propidium iodide stained nuclei.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Marcia Burch
Verified customer
Submitted Jul 09 2014