Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (primary neutrophils)
Permeabilization
Yes - 0.1% Triton in PBS
Specification
primary neutrophils
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 25°C
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fl
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Excellent for IF, used also with confocal laser scanning microscopy. We tested dilution 1/100 and 1/50, but better results at 1/50.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Jose Ramos Vivas
Verified customer
Submitted May 12 2016