Recombinant
RabMAb

Recombinant Anti-Neutrophil Elastase antibody [SP203] (ab183342)

Overview

  • Product name

    Anti-Neutrophil Elastase antibody [SP203]
    See all Neutrophil Elastase primary antibodies
  • Description

    Rabbit monoclonal [SP203] to Neutrophil Elastase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Neutrophil Elastase aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P08246

  • Positive control

    • IHC-P: Human tonsil, and spleen tissue; FC: THP-1 cells.
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.
IHC-P 1/100.

Target

  • Function

    Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
  • Tissue specificity

    Bone marrow cells.
  • Involvement in disease

    Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
    Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
  • Sequence similarities

    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bone marrow serine protease antibody
    • ELA2 antibody
    • ELANE antibody
    • Elastase 2 antibody
    • Elastase 2 neutrophil antibody
    • Elastase neutrophil expressed antibody
    • Elastase-2 antibody
    • ELNE_HUMAN antibody
    • GE antibody
    • Granulocyte derived elastase antibody
    • HLE antibody
    • HNE antibody
    • Human leukocyte elastase antibody
    • Leukocyte elastase antibody
    • Medullasin antibody
    • NE antibody
    • Neutrophil elastase antibody
    • PMN E antibody
    • PMN elastase antibody
    • Polymorphonuclear elastase antibody
    • SCN1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling Neutrophil Elastase with ab183342 at 1/100 dilution (2.46 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow cytometry analysis of THP-1 (human monocytic leukemia monocyte) labeling Neutrophil Elastase with purified ab183342 at 1/20 dilution (12.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
  • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Neutrophil Elastase with ab183342 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Neutrophil Elastase with ab183342 at 1/100 dilution.

References

ab183342 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

We have data showing ab183342 has positive staining in both neutrophils and monocytes.

Read More

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