Overview

  • Product name

    Neutrophil Elastase Inhibitor Assay Kit
    See all Neutrophil Elastase kits
  • Detection method

    Fluorescent
  • Assay type

    Quantitative
  • Assay time

    0h 40m
  • Product overview

    In Abcam's Neutrophil Elastase Inhibitor Screening Kit, NE hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent Neutrophil Elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of Neutrophil Elastase inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
    Visit our FAQs page for tips and troubleshooting.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Assay Buffer WM 1 x 25ml
    Inhibitor Control (3 mM, SPCK) Purple 1 x 100µl
    Neutrophil Elastase Green 1 vial
    Substrate Red 1 x 0.2ml
  • Research areas

  • Relevance

    Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage.
  • Alternative names

    • Bone marrow serine protease
    • ELA2
    • ELANE
    • Elastase 2
    • Elastase 2 neutrophil
    • Elastase neutrophil expressed
    • GE
    • Granulocyte derived elastase
    • HLE
    • HNE
    • Human leukocyte elastase
    • Leukocyte elastase
    • Medullasin
    • NE
    • PMN E
    • PMN elastase
    • Polymorphonuclear elastase
    • SCN1
    see all

Images

  • Neutrophil Elastase Activity Profile at Various Concentrations of Elafin/Skalp (ab153768)

  • Neutrophil Elastase Activity Profile at Various Concentrations of SERPINB1 (ab114774)

  • Neutrophil Elastase Inhibition by SPCK
  • Neutrophil Elastase Activity Profile at Various Concentrations of SPCK

Protocols

References

This product has been referenced in:

  • Goldberg EL  et al. ß-Hydroxybutyrate Deactivates Neutrophil NLRP3 Inflammasome to Relieve Gout Flares. Cell Rep 18:2077-2087 (2017). Read more (PubMed: 28249154) »
  • Eggenschwiler R  et al. Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase. Sci Rep 6:38198 (2016). Functional Studies . Read more (PubMed: 27910942) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

RFU is an arbitrary unit and is dependent on the specific instrument and the gain/sensitivity, so unfortunately there is no way to give you a generic RFU to nmole correlation for calculation.

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Answer

1. From what species is the elastase purified? Human.
2. What is the concentration of elastase provided? There is 15-22 mU of enzyme in the kit. The exact amount is proprietary.
3. What is the substrate? Eg. what is the sequence? MeOSuc - Ala - Ala - Pro - Val – AFC; MeOSuc-AAPV-AFC
4. What is the concentration of the substrate? We provide 4.5-6.2 mM concentration of substrate.


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Question
Answer

Yes. ab142178 is same as the substrate in ab118971.

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Answer

Thank you for contacting us.

The substrate that is in the kit will be cleaved by active elastase, whether pure, as provided in the kit, or in biological samples. You could use the pure elastase in the kit as a standard for comparison to your samples. However, the amount of elastase activity in your samples may be below the detection limit of the assay. We are not aware of any tests of biological samples.

Please let me know if you have any other questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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