Neutrophil Elastase Inhibitor Assay Kit (ab118971)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 40 min
Overview
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Product name
Neutrophil Elastase Inhibitor Assay Kit
See all Neutrophil Elastase kits -
Detection method
Fluorescent -
Assay type
Quantitative -
Assay time
0h 40m -
Product overview
In Abcam's Neutrophil Elastase Inhibitor Screening Kit, NE hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent Neutrophil Elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of Neutrophil Elastase inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K782 Neutrophil Elastase Inhibitor Screening Kit (Fluorometric). K782-100 is the same size as the 100 test size of ab118971.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer XL 1 x 25ml Elastase Inhibitor 1 x 100µl Elastase Enzyme 1 vial Substrate VI 1 x 0.2ml -
Research areas
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Relevance
Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage. -
Alternative names
- Bone marrow serine protease
- ELA2
- ELANE
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Images
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab118971 has been referenced in 13 publications.
- Bujak T et al. Flower Extracts as Multifunctional Dyes in the Cosmetics Industry. Molecules 27:N/A (2022). PubMed: 35164187
- Ziemlewska A et al. Evaluation of Cosmetic and Dermatological Properties of Kombucha-Fermented Berry Leaf Extracts Considered to Be By-Products. Molecules 27:N/A (2022). PubMed: 35408743
- Nowak A et al. Epilobium angustifolium L. Extracts as Valuable Ingredients in Cosmetic and Dermatological Products. Molecules 26:N/A (2021). PubMed: 34200200
- Zagórska-Dziok M et al. Positive Effect of Cannabis sativa L. Herb Extracts on Skin Cells and Assessment of Cannabinoid-Based Hydrogels Properties. Molecules 26:N/A (2021). PubMed: 33557174
- Bujak T et al. Antioxidant and Cytoprotective Properties of Plant Extract from Dry Flowers as Functional Dyes for Cosmetic Products. Molecules 26:N/A (2021). PubMed: 34068613