Product nameNeutrophil Marker (CD11b, CD16, CD32, CD44, CD55) Antibody Panel - Human
Species reactivityReacts with: Human
Neutrophil Marker (CD11b, CD16, CD32, CD44, CD55) Antibody Panel - Human ab254016 contains multiple trial-sized versions of anti-human antibody clones against CD11b, CD16, CD32, CD44, CD55, specifically selected for high performance in various applications. This panel contains 3 recombinant rabbit monoclonal antibodies against human CD11b, CD16, CD55 and 2 mouse monoclonal antibodies against human CD32, CD44. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
- Rabbit monoclonal [SP330] to CD11b (20 µL) ab224805
- Rabbit monoclonal [SP175] to CD16 (20 µL) ab183354
- Mouse monoclonal [AT10] to CD32 (20 µg) ab23336
- Mouse monoclonal [F10-44-2] to CD44 (20 µg) ab6124
- Rabbit monoclonal [EPR6689] to CD55 (20 µL) ab133684
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsPlease refer to protocols.
Components 1 kit Anti-CD11b antibody [SP330] - C-terminal (ab224805) 2 x 10µl Anti-CD16 antibody [SP175] (ab183354) 2 x 10µl Anti-CD32 antibody [AT10] (ab23336) 2 x 10µg Anti-CD44 antibody [F10-44-2] (ab6124) 2 x 10µg Anti-CD55 antibody [EPR6689] (ab133684) 2 x 10µl
Cellular localizationCD44: Membrane. CD55: Cell membrane. CD11b: Membrane. CD16: Cell membrane. Secreted. Exists also as a soluble receptor. CD32: Cell membrane.
- Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797)
- Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
- Anti-CD16 antibody [SP175] - BSA and Azide free (ab243925)
- Anti-CD32B + CD32A antibody [AT10] (FITC) (ab30356)
- Anti-CD32B + CD32A antibody [AT10] (PE) (ab30357)
- Anti-CD44 antibody [F10-44-2] (FITC) (ab30405)
- Anti-CD32B + CD32A antibody [AT10] - BSA free (ab41899)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling CD55 with ab133684 at 1/100 dilution.
Immunohistochemical analysis of paraffin embedded human spleen tissue labeling CD16 with ab183354 at 1/100 dilution.
ab6412 staining CD44 in A431 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab6142 at 5ugml then detected with an Alexa Fluor® 488 goat anti-mouse secondary antibody (ab150117) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab202272, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).
IHC image of CD44 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6124, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254016 has not yet been referenced specifically in any publications.