Anti-Nexilin/F-actin-binding protein antibody (ab233235)
Key features and details
- Rabbit polyclonal to Nexilin/F-actin-binding protein
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-Nexilin/F-actin-binding protein antibody
See all Nexilin/F-actin-binding protein primary antibodies -
Description
Rabbit polyclonal to Nexilin/F-actin-binding protein -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Rat Nexilin/F-actin-binding protein aa 200-500. N-terminal tags. Expressed in E. coli.
Database link: Q9Z2J4-2 -
Positive control
- IHC-P: Rat cardiac muscle tissue. WB: Recombinant rat Nexilin/F-actin-binding protein. Rat heart, lung, cerebrum and testis tissue lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 78 kDa.
|
Notes |
---|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 78 kDa. |
Target
-
Function
Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity. -
Tissue specificity
Abundantly expressed in heart and skeletal muscle, and at lower levels in placenta, lung, liver and pancreas. Also expressed in HeLa S3 and Molt-4 cells. -
Involvement in disease
Defects in NEXN are the cause of cardiomyopathy dilated type 1CC (CMD1CC) [MIM:613122]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in NEXN are the cause of cardiomyopathy familial hypertrophic type 20 (CMH20) [MIM:613876]. CMH20 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cytoplasm > myofibril > sarcomere > Z line. Localizes to the cell-matrix AJ. Not found at the cell-cell AJ. - Information by UniProt
-
Database links
- Entrez Gene: 68810 Mouse
- Entrez Gene: 246172 Rat
- SwissProt: Q7TPW1 Mouse
- SwissProt: Q9Z2J4 Rat
- Unigene: 200188 Mouse
- Unigene: 107975 Rat
-
Alternative names
- 1110046H09Rik antibody
- AA553326 antibody
- F-actin binding protein antibody
see all
Images
-
Formalin-fixed, paraffin-embedded rat cardiac muscle tissue stained for Nexilin/F-actin-binding protein with ab233235 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/ml + Rat testis tissue lysate
Predicted band size: 78 kDa -
Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/ml + Rat cerebrum tissue lysate
Predicted band size: 78 kDa -
Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/ml + Rat lung tissue lysate
Predicted band size: 78 kDa -
Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/ml + Rat heart tissue lysate
Predicted band size: 78 kDa -
Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/ml + Recombinant rat Nexilin/F-actin-binding protein
Predicted band size: 78 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab233235 has been referenced in 2 publications.
- Huang J et al. CDC-like kinase 4 deficiency contributes to pathological cardiac hypertrophy by modulating NEXN phosphorylation. Nat Commun 13:4433 (2022). PubMed: 35907876
- Hu YW et al. Long non-coding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN. J Clin Invest N/A:N/A (2018). PubMed: 30589415