Key features and details
- Rabbit polyclonal to Nexilin/F-actin-binding protein
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nexilin/F-actin-binding protein antibody
See all Nexilin/F-actin-binding protein primary antibodies
DescriptionRabbit polyclonal to Nexilin/F-actin-binding protein
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
Protein previously labeled as Nexilin.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab83746 is purified by a peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab83746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionInvolved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity.
Tissue specificityAbundantly expressed in heart and skeletal muscle, and at lower levels in placenta, lung, liver and pancreas. Also expressed in HeLa S3 and Molt-4 cells.
Involvement in diseaseDefects in NEXN are the cause of cardiomyopathy dilated type 1CC (CMD1CC) [MIM:613122]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in NEXN are the cause of cardiomyopathy familial hypertrophic type 20 (CMH20) [MIM:613876]. CMH20 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > adherens junction. Cytoplasm > myofibril > sarcomere > Z line. Localizes to the cell-matrix AJ. Not found at the cell-cell AJ.
- Information by UniProt
- 1110046H09Rik antibody
- AA553326 antibody
- F-actin binding protein antibody
Anti-Nexilin/F-actin-binding protein antibody (ab83746) at 1 µg/ml (in 5% skim milk/ PBS) + Fetal liver lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 81 kDa
Observed band size: 81 kDa
Additional bands at: 39 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
ab83746 has been referenced in 3 publications.
- Hu YW et al. Long non-coding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN. J Clin Invest N/A:N/A (2018). PubMed: 30589415
- Barbati SA et al. Transcription Factor CREM Mediates High Glucose Response in Cardiomyocytes and in a Male Mouse Model of Prolonged Hyperglycemia. Endocrinology 158:2391-2405 (2017). PubMed: 28368536
- Soni S et al. A Proteomics Approach to Identify New Putative Cardiac Intercalated Disk Proteins. PLoS One 11:e0152231 (2016). IHC . PubMed: 27148881