Overview

  • Product name

    Anti-NF-kB p65 (phospho S276) antibody
    See all NF-kB p65 primary antibodies
  • Description

    Rabbit polyclonal to NF-kB p65 (phospho S276)
  • Host species

    Rabbit
  • Specificity

    ab106129 is specific for phosphorylated S276 NF-kB p65. It shows minimal reactivity by Western blot with non-phosphorylated NFkB p65, and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
  • Tested applications

    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human NF-kB p65 conjugated to keyhole limpet haemocyanin. Corresponding to a region near phospho Serine 276 of the human protein.

  • Positive control

    • WB: TNF alpha stimulated HeLa whole cell lysate (ab150035); TNF inducible KBM5 cell lysates.
  • General notes

    Dilute only prior to immediate use.
    Expiration date is one (1) year from date of opening.

Properties

Applications

Our Abpromise guarantee covers the use of ab106129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 60 kDa.
ELISA 1/5000 - 1/25000.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications

    Ubiquitinated, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.
    Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes.
    Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity.
    Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of 'Lys-310'.
  • Cellular localization

    Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.
  • Information by UniProt
  • Database links

  • Alternative names

    • Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
    • MGC131774 antibody
    • NF kappa B p65delta3 antibody
    • nfkappabp65 antibody
    • NFkB p65 antibody
    • NFKB3 antibody
    • Nuclear factor kappaB antibody
    • Nuclear Factor NF Kappa B p65 Subunit antibody
    • Nuclear factor NF-kappa-B p65 subunit antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
    • OTTHUMP00000233473 antibody
    • OTTHUMP00000233474 antibody
    • OTTHUMP00000233475 antibody
    • OTTHUMP00000233476 antibody
    • OTTHUMP00000233900 antibody
    • p65 antibody
    • p65 NF kappaB antibody
    • p65 NFkB antibody
    • relA antibody
    • TF65_HUMAN antibody
    • Transcription factor NFKB3 antibody
    • Transcription factor p65 antibody
    • v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody
    • V rel avian reticuloendotheliosis viral oncogene homolog A antibody
    • v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody
    • V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody
    see all

Images

  • All lanes : Anti-NF-kB p65 (phospho S276) antibody (ab106129) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates, stimulated with TNF alpha

    Predicted band size: 60 kDa

References

This product has been referenced in:

  • Hou M  et al. Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. Infect Immun 87:N/A (2019). Read more (PubMed: 30396897) »
  • Li H & Chen C Quercetin Has Antimetastatic Effects on Gastric Cancer Cells via the Interruption of uPA/uPAR Function by Modulating NF-?b, PKC-d, ERK1/2, and AMPKa. Integr Cancer Ther 17:511-523 (2018). Read more (PubMed: 28627240) »
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary hippocampal neurons)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Primary hippocampal neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 30 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
Yes - Triton x-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 26 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (UVB-induced murine skin tissue)
Specification
UVB-induced murine skin tissue
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Mar 18 2013

Answer

Thank you for your reply.
Just to make sure there is no confusion, ab30623 & ab106129 are both NFkB phosphoS276 antibodies, not Re1A. Is this just an alternative name?
If you are looking for an antibody against NFkB phosphoS276, then I would recommend ab30623, as it is guaranteed to work in WB, IHC-P, ELISA & IP, in human, rat and pig samples.
If you would like to use the antibody in any other species or application then please let me know, as you could qualify for our Testing Discount Program:
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting Abcam.
As we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our you that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.
If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased.
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
Please contact our Scientific Support team by replying to this email prior to purchase for more information.
Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
To find out more about our Abreview system, please see the following link:
https://www.abcam.com/abreviews
I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Answer

DISCOUNT CODE: *****
Expiration date: *****

I am very pleased to hear you would like to accept our offer and test ab106129 in IHC and mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us.

ab106129 has not been tested in IHC-P or mouse sections, therefore we do not know whether this product is suitable. I can offer a discount off a future purchase if you buy ab106129 now, test it in IHC and mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody off your next order.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab106129 in IHC and mouse. I will then send a discount code. This code must be issued before purchasing ab106129 so please wait for my reply before ordering.

2. Purchase ab106129 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC and mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab106129 turns out to be unsuitable for IHC or mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. You might want to consider testing mouse samples in WB first to identify whether it is suitable for this species as WB is already a tested application. We would welcome an Abreview for this as well.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Application
Western blot
Sample
Rat Cell lysate - whole cell (Spinal cord tissue)
Loading amount
20 µg
Specification
Spinal cord tissue
Gel Running Conditions
Reduced Denaturing (4-20% Tris-glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Ms. Kelly Atkins

Verified customer

Submitted Jun 18 2012

Question
Answer

DISCOUNT CODE: ***
Expiration date: August 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab106129 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your inquiry.

I sincerely apologise for the inconvenience the long delay in availability of ab30623 is causing you.

We do offer an alternative antibody that is also specific for the phosphorylation site S276. Unfortunately this antibody has a lead time of 1-2 weeks at the moment.

ab106129 (Anti-NFkB p65 (phospho S276) antibody)

https://www.abcam.com/index.html?datasheet=106129 (or use the following: https://www.abcam.com/index.html?datasheet=106129).

It is not tested and guaranteed for zebra fish. But I am confident that it will also work if the phosphorylation site S276 is conserved in zebrafish.

I am very sorry that I do not have a positive answer for you on this occasion and hope this information is nevertheless helpful.


I wish you all the best with your publication.

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