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    nf-kb-p65-phospho-s276-antibody-ab194726.pdf

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Cell Biology Apoptosis Intracellular NFkB RelA (p65)
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Anti-NF-kB p65 (phospho S276) antibody (ab194726)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (17)

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Western blot - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)

Key features and details

  • Rabbit polyclonal to NF-kB p65 (phospho S276)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-NF-kB p65 (phospho S276) antibody [EPR17622] (ab183559)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-NF-kB p65 (phospho S276) antibody
    See all NF-kB p65 primary antibodies
  • Description

    Rabbit polyclonal to NF-kB p65 (phospho S276)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human NF-kB p65 (phospho S276). The exact sequence is proprietary.
    Database link: Q04206

  • Positive control

    • WB: HeLa and C6 cells treated with TNF-a lysates; IHC-P: Human breast, breast cancer and rat testis tissues; ICC/IF: U-2OS cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • RelA (p65)
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Microbiology
    • Interspecies Interaction
    • Host Immune Response
    • Metabolism
    • Types of disease
    • Obesity
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • RelA (p65)

Associated products

  • Assay kits

    • NFkB p65 Transcription Factor Assay Kit (ab133112)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human NF-kB p65 protein (Tagged) (ab114150)
  • Related Products

    • NF kappaB p65 (pS536) ELISA Kit (ab176647)
    • NF kappaB p65 (pS536 + Total) ELISA Kit (ab176663)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/500 - 1/2000. Predicted molecular weight: 60 kDa.
IHC-P
1/50 - 1/100.
ICC/IF
1/20 - 1/50.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 60 kDa.
IHC-P
1/50 - 1/100.
ICC/IF
1/20 - 1/50.

Target

  • Function

    NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications

    Ubiquitinated, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.
    Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes.
    Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity.
    Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of 'Lys-310'.
  • Cellular localization

    Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.
  • Target information above from: UniProt accession Q04206 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5970 Human
    • Entrez Gene: 309165 Rat
    • Omim: 164014 Human
    • SwissProt: Q04206 Human
    • Unigene: 502875 Human
    • Alternative names

      • Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
      • MGC131774 antibody
      • NF kappa B p65delta3 antibody
      • nfkappabp65 antibody
      • NFkB p65 antibody
      • NFKB3 antibody
      • Nuclear factor kappaB antibody
      • Nuclear Factor NF Kappa B p65 Subunit antibody
      • Nuclear factor NF-kappa-B p65 subunit antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
      • OTTHUMP00000233473 antibody
      • OTTHUMP00000233474 antibody
      • OTTHUMP00000233475 antibody
      • OTTHUMP00000233476 antibody
      • OTTHUMP00000233900 antibody
      • p65 antibody
      • p65 NF kappaB antibody
      • p65 NFkB antibody
      • relA antibody
      • TF65_HUMAN antibody
      • Transcription factor NFKB3 antibody
      • Transcription factor p65 antibody
      • v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody
      • V rel avian reticuloendotheliosis viral oncogene homolog A antibody
      • v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody
      • V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody
      see all

    Images

    • Western blot - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
      Western blot - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
      Anti-NF-kB p65 (phospho S276) antibody (ab194726) + HeLa cell extract at 25 µg

      Secondary
      HRP Goat Anti-Rabbit IgG.

      Predicted band size: 60 kDa



      HeLa cells were treated by TNF-α (20ng/ml) for 30 minutes.

      Blocking buffer: 3% BSA.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling NF-kB p65 using ab194726 at 1/100 dilution. 

    • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
      Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 (phospho S276) antibody (ab194726)

      Immunocytochemistry/Immunofluorescent analysis of U-20S cells staining NF-kB p65 with ab194726 at 1/100 dilution. DAPI was used as a nuclear counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)

      Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling NF-kB p65 using ab194726 at 1/100 dilution. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling NF-kB p65 using ab194726 at 1/100 dilution. 

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (17)

    Publishing research using ab194726? Please let us know so that we can cite the reference in this datasheet.

    ab194726 has been referenced in 17 publications.

    • Wang G  et al. Suppression of PAPP-A mitigates atherosclerosis by mediating macrophage polarization via STAT3 signaling. Biochem Biophys Res Commun 543:29-37 (2021). PubMed: 33508770
    • Li J  et al. The MIR155 host gene/microRNA-627/HMGB1/NF-?B loop modulates fibroblast proliferation and extracellular matrix deposition. Life Sci 269:119085 (2021). PubMed: 33482190
    • Liu X  et al. Effects of Curcumin on High Glucose-Induced Epithelial-to-Mesenchymal Transition in Renal Tubular Epithelial Cells Through the TLR4-NF-?B Signaling Pathway. Diabetes Metab Syndr Obes 14:929-940 (2021). PubMed: 33688227
    • Gan YR  et al. Dickkopf-1/cysteine-rich angiogenic inducer 61 axis mediates palmitic acid-induced inflammation and apoptosis of vascular endothelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33300071
    • Mou SQ  et al. Liquiritin Attenuates Lipopolysaccharides-Induced Cardiomyocyte Injury via an AMP-Activated Protein Kinase-Dependent Signaling Pathway. Front Pharmacol 12:648688 (2021). PubMed: 34054527
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Western blot abreview for Anti-NF-kB p65 (phospho S276) antibody

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    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Balb/C mice brain)
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-glycin gel)
    Loading amount
    40 µg
    Specification
    Balb/C mice brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 30 2020

    Western blot abreview for Anti-NF-kB p65 (phospho S276) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HMC3 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-glycin gel)
    Loading amount
    40 µg
    Specification
    HMC3 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 30 2020

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