Product nameNF-kB Signaling Pathway Antibody Sampler Panel
ab228529, NF-kB Signaling Pathway Antibody Panel, is a collection of 1 anti-rabbit (HRP) secondary antibody and 5 recombinant rabbit monoclonal antibodies against IKB alpha (p S36), IKB alpha, NFkB p105, NF-kB p65 (p S536), and NF-kB p65 provided in 10 µl trial sizes.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit ab133462 - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] 1 x 10µl ab32518 - Anti-IKB alpha antibody [E130] 1 x 10µl ab32360 - Anti-NFkB p105 / p50 antibody [E381] 1 x 10µl ab76302 - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] 1 x 10µl ab32536 - Anti-NF-kB p65 antibody [E379] 1 x 10µl ab205718 - Goat Anti-Rabbit IgG H+L (HRP) 1 x 100µg
- Anti-NF-kB p65 antibody [E379] (Alexa Fluor® 488) (ab190205)
- Anti-NF-kB p65 antibody [E379] (Alexa Fluor® 647) (ab190589)
- Anti-IKB alpha antibody [E130] (Alexa Fluor® 488) (ab202644)
- Anti-IKB alpha antibody [E130] (Alexa Fluor® 647) (ab202647)
- Anti-IKB alpha antibody [E130] (Alexa Fluor® 594) (ab206346)
- Anti-IKB alpha antibody [E130] (Alexa Fluor® 555) (ab206578)
- Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)
- Anti-NF-kB p65 antibody [E379] (Alexa Fluor® 594) (ab214846)
- Anti-IKB alpha antibody [E130] - BSA and Azide free (ab215972)
- Anti-NFkB p105 / p50 antibody [E381] - BSA and Azide free (ab220803)
- Anti-IKB alpha antibody [E130] (APC) (ab224948)
- Anti-IKB alpha antibody [E130] (PE) (ab224949)
- Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
- Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)
ICC/IF image of unpurified ab32518 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32518, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Predicted band: 105 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: NFkB p105 / p50 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
ab32360 was shown to specifically react with NFkB p105 / p50 when NFkB p105 / p50 knockout samples were used. Wild-type and NFkB p105 / p50 knockout samples were subjected to SDS-PAGE. ab32360 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of colon sections from mice, staining NF-kB p65 with ab32536.
Antigen retrieval was performed by microwave heating in citrate buffer, pH 6. Sections were incubated overnight with primary antibody (1/250) and staining was detected using ab80437 EXPOSE Rabbit specific HRP/DAB detection IHC kit.
Primary - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) at 1/10000 dilution
Lane 1: HeLa cell lysate, 10 µg
Lane 2: HeLa cell lysate treated with FBS and Calyculin A, 10 µg
Secondary - HRP labeled goat anti-rabbit IgG, 1/2000 dilution
Predicted band: 35 kDa
Actual band: 40 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228529 has not yet been referenced specifically in any publications.