Anti-NFAT1 antibody (ab150330)

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Overview

  • Product name

    Anti-NFAT1 antibody
    See all NFAT1 primary antibodies

  • Description

    Rabbit polyclonal to NFAT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Chinese hamster

  • Immunogen

    Synthetic peptide within Mouse NFAT1 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q60591

  • Positive control

    • This antibody gave a positive signal in BC3H1 whole cell lysate as well as the following Mouse tissue lysates: Brain; Placenta; Pancreas.

Properties

Applications

Our Abpromise guarantee covers the use of ab150330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

Target

  • Function

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.

  • Tissue specificity

    Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.

  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.

  • Domain

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.

  • Post-translational
    modifications

    In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI607462 antibody
      • cytoplasmic 2 antibody
      • KIAA0611 antibody
      • NF ATc2 antibody
      • NF ATp antibody
      • NF-ATc2 antibody
      • NF-ATp antibody
      • NFAC2_HUMAN antibody
      • NFAT 1 antibody
      • NFAT pre existing subunit antibody
      • NFAT pre-existing subunit antibody
      • NFAT transcription complex, preexisting component antibody
      • NFAT1 antibody
      • NFAT1-D antibody
      • NFATc2 antibody
      • NFATp antibody
      • Nuclear factor of activated T cells cytoplasmic 2 antibody
      • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
      • Nuclear factor of activated T cells pre-existing component antibody
      • Nuclear factor of activated T cells, preexisting component antibody
      • Nuclear factor of activated T-cells antibody
      • Preexisting nuclear factor of activated T cells 2 antibody
      • T cell transcription factor NFAT 1 antibody
      • T-cell transcription factor NFAT1 antibody
      see all

    Images

    • Western blot - Anti-NFAT1 antibody (ab150330)
      Western blot - Anti-NFAT1 antibody (ab150330)
      All lanes : Anti-NFAT1 antibody (ab150330) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Pancreas (Mouse) Tissue Lysate
      Lane 3 : Mouse Placenta Tissue Lysate
      Lane 4 : BC3H1 Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 100 kDa
      Observed band size: 100 kDa
      Additional bands at: 70 kDa (possible non-specific binding)


      Exposure time: 4 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab150330 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    References

    ab150330 has not yet been referenced specifically in any publications.

    Publishing research using ab150330? Please let us know so that we can cite the reference in this datasheet.

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