Overview

  • Product name

  • Description

    Goat polyclonal to NFAT1
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognize all three reported isoforms (NP_036472.2; NP_775114.1; NP_001129493.1).
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    QQFRTGLSSPDARYQ

    by a Cysteine residue linker, corresponding to internal sequence amino acids 729 - 743 of Human NFAT1 (NP_036472.2; NP_775114.1; NP_001129493.1)

  • Positive control

    • MOLT4 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab95479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).

Target

  • Function

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
  • Tissue specificity

    Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.
  • Domain

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications

    In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI607462 antibody
    • cytoplasmic 2 antibody
    • KIAA0611 antibody
    • NF ATc2 antibody
    • NF ATp antibody
    • NF-ATc2 antibody
    • NF-ATp antibody
    • NFAC2_HUMAN antibody
    • NFAT 1 antibody
    • NFAT pre existing subunit antibody
    • NFAT pre-existing subunit antibody
    • NFAT transcription complex, preexisting component antibody
    • NFAT1 antibody
    • NFAT1-D antibody
    • NFATc2 antibody
    • NFATp antibody
    • Nuclear factor of activated T cells cytoplasmic 2 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
    • Nuclear factor of activated T cells pre-existing component antibody
    • Nuclear factor of activated T cells, preexisting component antibody
    • Nuclear factor of activated T-cells antibody
    • Preexisting nuclear factor of activated T cells 2 antibody
    • T cell transcription factor NFAT 1 antibody
    • T-cell transcription factor NFAT1 antibody
    see all

Images

  • Anti-NFAT1 antibody (ab95479) at 0.3 µg/ml + MOLT4 lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 100 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.
  • ab95479 at 3.8 µg/ml staining NFAT1 in Human spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections. ed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab95479 has not yet been referenced specifically in any publications.

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