Recombinant
RabMAb

Anti-NFAT1 antibody [EPR2973] (ab92490)

Submit an Abreview Q&A (2)

Overview

  • Product name
    Anti-NFAT1 antibody [EPR2973]
    See all NFAT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2973] to NFAT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human NFAT1 (UniProt Q13469).

  • Positive control
    • Jurkat cell lysate, Ramos cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa).

    Is unsuitable for ICC, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
    • Tissue specificity
      Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
    • Sequence similarities
      Contains 1 RHD (Rel-like) domain.
    • Domain
      Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
    • Post-translational
      modifications
      In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
    • Information by UniProt
    • Database links
      • Alternative names
        • AI607462 antibody
        • cytoplasmic 2 antibody
        • KIAA0611 antibody
        • NF ATc2 antibody
        • NF ATp antibody
        • NF-ATc2 antibody
        • NF-ATp antibody
        • NFAC2_HUMAN antibody
        • NFAT 1 antibody
        • NFAT pre existing subunit antibody
        • NFAT pre-existing subunit antibody
        • NFAT transcription complex, preexisting component antibody
        • NFAT1 antibody
        • NFAT1-D antibody
        • NFATc2 antibody
        • NFATp antibody
        • Nuclear factor of activated T cells cytoplasmic 2 antibody
        • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
        • Nuclear factor of activated T cells pre-existing component antibody
        • Nuclear factor of activated T cells, preexisting component antibody
        • Nuclear factor of activated T-cells antibody
        • Preexisting nuclear factor of activated T cells 2 antibody
        • T cell transcription factor NFAT 1 antibody
        • T-cell transcription factor NFAT1 antibody
        see all

      Images

      • Western blot - Anti-NFAT1 antibody [EPR2973] (ab92490)
        Western blot - Anti-NFAT1 antibody [EPR2973] (ab92490)
        All lanes : Anti-NFAT1 antibody [EPR2973] (ab92490) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : Ramos cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 100 kDa
        Observed band size: 135 kDa (why is the actual band size different from the predicted?)

      References

      ab92490 has not yet been referenced specifically in any publications.

      Publishing research using ab92490? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      2 reviews or Q&A

      10
      Votes: Highest First

      Yes, we would like to test this antibody for CHIP. Please send the promo code and we will process our order.

      I am very pleased to hear you would like to accept our offer and test ab92490 in ChIP. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for ChIP. The code is inac...

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      Would this antibody work for ChIP? Do you recommend other NFAT abs for ChIP?

      Thank you very much for your interest in ab92490.

      To our knowledge, this product nor any of our other anti-NFAT products, have not been tested in ChIP. However by participating in our AbTrial program, you can now use our products in an unt...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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