RecombinantRabMAb

Recombinant Anti-NFAT1 antibody [EPR2973] (ab92490)

Submit a review Q&A (2)References (1)
Western blot - Anti-NFAT1 antibody [EPR2973] (ab92490)
  • Anti-NFAT1 antibody [EPR2973] (ab92490)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2973] to NFAT1
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NFAT1 antibody [EPR2973]
    See all NFAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2973] to NFAT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human NFAT1 (UniProt Q13469).

  • Positive control

    • Jurkat cell lysate, Ramos cell lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab92490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa).

    Is unsuitable for ICC, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.

    • Tissue specificity

      Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.

    • Sequence similarities

      Contains 1 RHD (Rel-like) domain.

    • Domain

      Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.

    • Post-translational
      modifications

      In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.

    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
    • Information by UniProt

    • Database links

      • Alternative names

        • AI607462 antibody
        • cytoplasmic 2 antibody
        • KIAA0611 antibody
        • NF ATc2 antibody
        • NF ATp antibody
        • NF-ATc2 antibody
        • NF-ATp antibody
        • NFAC2_HUMAN antibody
        • NFAT 1 antibody
        • NFAT pre existing subunit antibody
        • NFAT pre-existing subunit antibody
        • NFAT transcription complex, preexisting component antibody
        • NFAT1 antibody
        • NFAT1-D antibody
        • NFATc2 antibody
        • NFATp antibody
        • Nuclear factor of activated T cells cytoplasmic 2 antibody
        • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
        • Nuclear factor of activated T cells pre-existing component antibody
        • Nuclear factor of activated T cells, preexisting component antibody
        • Nuclear factor of activated T-cells antibody
        • Preexisting nuclear factor of activated T cells 2 antibody
        • T cell transcription factor NFAT 1 antibody
        • T-cell transcription factor NFAT1 antibody
        see all

      Images

      • Western blot - Anti-NFAT1 antibody [EPR2973] (ab92490)
        Western blot - Anti-NFAT1 antibody [EPR2973] (ab92490)
        All lanes : Anti-NFAT1 antibody [EPR2973] (ab92490) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : Ramos cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 100 kDa
        Observed band size: 135 kDa
        why is the actual band size different from the predicted?

      • Anti-NFAT1 antibody [EPR2973] (ab92490)
        Anti-NFAT1 antibody [EPR2973] (ab92490)

      References (1)

      Publishing research using ab92490? Please let us know so that we can cite the reference in this datasheet.

      ab92490 has been referenced in 1 publication.

      • Zhang Y  et al. ASIC1a induces synovial inflammation via the Ca2+/NFATc3/ RANTES pathway. Theranostics 10:247-264 (2020). PubMed: 31903118

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Yes, we would like to test this antibody for CHIP. Please send the promo code and we will process our order.

      Read More
      Answer

      I am very pleased to hear you would like to accept our offer and test ab92490 in ChIP. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for ChIP. The code is inactive until included in your submitted Abreview so be sure to add it to the Additional Comments section. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/AbTrial

      Read More

      Question

      Would this antibody work for ChIP? Do you recommend other NFAT abs for ChIP?

      Read More
      Answer

      Thank you very much for your interest in ab92490.

      To our knowledge, this product nor any of our other anti-NFAT products, have not been tested in ChIP. However by participating in our AbTrial program, you can now use our products in an untested application or species without financial risk.

      Simply follow these easy steps below to take part in our AbTrial Program:

      1. Reply to this e-mail to let me know that you would like to proceed and test this product.

      2. I will email you an inactive personal discount code for the value of the product.

      3. Purchase and test the product at regular price.

      4. https://www.abcam.com/abreviews with your results. Note your AbTrial discount code in the additional notes section.

      5. Once the Abreview is submitted, the discount code will become active.

      6. Apply your discount code on your next order to receive that value off.

      More information including the terms and conditions about this program may be found at: https://www.abcam.com/AbTrial

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com