Key features and details
- Rabbit polyclonal to NFAT1 (phospho S54)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-NFAT1 (phospho S54) antibody
See all NFAT1 primary antibodies
DescriptionRabbit polyclonal to NFAT1 (phospho S54)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse NFAT1 (phospho S54).
Database link: Q60591
- Murine T-cells + PMA + Ionomycin.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab200819 was negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated NFAT1. The final product is generated by affinity chromatography using a NFAT1-derived peptide phosphorylated at serine 54.
Our Abpromise guarantee covers the use of ab200819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.|
FunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
Tissue specificityExpressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
Sequence similaritiesContains 1 RHD (Rel-like) domain.
DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
modificationsIn resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
- Information by UniProt
- AI607462 antibody
- cytoplasmic 2 antibody
- KIAA0611 antibody
All lanes : Anti-NFAT1 (phospho S54) antibody (ab200819) at 1/1000 dilution
Lane 1 : Extracts of Murine T cells were left untreated with no peptide
Lane 2 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with no peptide
Lane 3 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with the non-phosphopeptide corresponding to the phosphopeptide immunogen
Lane 4 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with a generic phosphoserine-containing peptide
Lane 5 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with the phosphopeptide immunogen
All lanes : Goat F(ab’)2 anti-rabbit IgG alkaline phosphatase
Predicted band size: 100 kDa
Resolved by SDS-PAGE on a 10% Tris-glycine gel
ab200819 has been referenced in 3 publications.
- Wu L et al. FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis. EBioMedicine 41:384-394 (2019). PubMed: 30803933
- Molinari F et al. The mitochondrial metabolic reprogramming agent trimetazidine as an 'exercise mimetic' in cachectic C26-bearing mice. J Cachexia Sarcopenia Muscle 8:954-973 (2017). PubMed: 29130633
- Zhang X et al. Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells. Mol Cancer 16:147 (2017). PubMed: 28851360