• Product name

    Anti-NFAT1 (phospho S54) antibody
    See all NFAT1 primary antibodies
  • Description

    Rabbit polyclonal to NFAT1 (phospho S54)
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse NFAT1 (phospho S54).
    Database link: Q60591

  • Positive control

    • Murine T-cells + PMA + Ionomycin.



Our Abpromise guarantee covers the use of ab200819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.


  • Function

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
  • Tissue specificity

    Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.
  • Domain

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational

    In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI607462 antibody
    • cytoplasmic 2 antibody
    • KIAA0611 antibody
    • NF ATc2 antibody
    • NF ATp antibody
    • NF-ATc2 antibody
    • NF-ATp antibody
    • NFAC2_HUMAN antibody
    • NFAT 1 antibody
    • NFAT pre existing subunit antibody
    • NFAT pre-existing subunit antibody
    • NFAT transcription complex, preexisting component antibody
    • NFAT1 antibody
    • NFAT1-D antibody
    • NFATc2 antibody
    • NFATp antibody
    • Nuclear factor of activated T cells cytoplasmic 2 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
    • Nuclear factor of activated T cells pre-existing component antibody
    • Nuclear factor of activated T cells, preexisting component antibody
    • Nuclear factor of activated T-cells antibody
    • Preexisting nuclear factor of activated T cells 2 antibody
    • T cell transcription factor NFAT 1 antibody
    • T-cell transcription factor NFAT1 antibody
    see all


  • All lanes : Anti-NFAT1 (phospho S54) antibody (ab200819) at 1/1000 dilution

    Lane 1 : Extracts of Murine T cells were left untreated with no peptide
    Lane 2 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
    ionomycin with no peptide
    Lane 3 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
    ionomycin with the non-phosphopeptide corresponding to the phosphopeptide immunogen
    Lane 4 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
    ionomycin with a generic phosphoserine-containing peptide
    Lane 5 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
    ionomycin with the phosphopeptide immunogen

    All lanes : Goat F(ab’)2 anti-rabbit IgG alkaline phosphatase

    Predicted band size: 100 kDa

    Resolved by SDS-PAGE on a 10% Tris-glycine gel


This product has been referenced in:

  • Wu L  et al. FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis. EBioMedicine 41:384-394 (2019). Read more (PubMed: 30803933) »
  • Molinari F  et al. The mitochondrial metabolic reprogramming agent trimetazidine as an 'exercise mimetic' in cachectic C26-bearing mice. J Cachexia Sarcopenia Muscle 8:954-973 (2017). Read more (PubMed: 29130633) »
See all 3 Publications for this product

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