Anti-NFAT2 antibody (ab25916)
Key features and details
- Rabbit polyclonal to NFAT2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human, Chimpanzee
- Isotype: IgG
Overview
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Product name
Anti-NFAT2 antibody
See all NFAT2 primary antibodies -
Description
Rabbit polyclonal to NFAT2 -
Host species
Rabbit -
Specificity
The amino acid sequence used is 100% identical in human NFATc1A, B, C and E isoforms. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon the cell lines. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Chimpanzee -
Immunogen
Synthetic peptide:
NKRKYSLNGRQPPYSPHHS
, corresponding to amino acids 263-282 of Human NFATc1 -
Positive control
- Ramos cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
1/20.
|
| WB | (4) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight varies with isoform.
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| Notes |
|---|
|
ICC/IF
1/20. |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight varies with isoform. |
Target
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Function
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. -
Tissue specificity
Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. -
Sequence similarities
Contains 1 RHD (Rel-like) domain. -
Domain
Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor. -
Post-translational
modificationsPhosphorylated by NFATC-kinase; dephosphorylated by calcineurin. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. - Information by UniProt
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Database links
- Entrez Gene: 4772 Human
- Entrez Gene: 18018 Mouse
- Omim: 600489 Human
- SwissProt: O95644 Human
- SwissProt: O88942 Mouse
- Unigene: 534074 Human
- Unigene: 701518 Human
- Unigene: 329560 Mouse
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Alternative names
- cytoplasmic 1 antibody
- MGC138448 antibody
- NF ATc antibody
see all
Images
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Western blot - Anti-NFAT2 antibody (ab25916)All lanes : Anti-NFAT2 antibody (ab25916) at 1 mg/ml
Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate -
Immunocytochemistry/ Immunofluorescence - Anti-NFAT2 antibody (ab25916)RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for NFAT2 (green) using ab25916 at 1/500 dilution in ICC/IF.
The nuclear counterstain is DAPI (blue). Alpha-Tubulin is stained with Dylight 550 (red).
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Western blot - Anti-NFAT2 antibody (ab25916)Western blot analysis of NFATc1 in Ramos cell lysate. Lane 1. Without blocking peptide. Lane 2. With blocking peptide.
Protocols
Datasheets and documents
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Datasheet download
References (35)
ab25916 has been referenced in 35 publications.
- Chen X et al. LY3023414 inhibits both osteogenesis and osteoclastogenesis through the PI3K/Akt/GSK3 signalling pathway. Bone Joint Res 10:237-249 (2021). PubMed: 33789427
- Li N et al. Inhibition of Sirtuin 3 prevents titanium particle-induced bone resorption and osteoclastsogenesis via suppressing ERK and JNK signaling. Int J Biol Sci 17:1382-1394 (2021). PubMed: 33867853
- Jia X et al. Overexpression of miRNA-22-3p attenuates osteoporosis by targeting MAPK14. Exp Ther Med 22:692 (2021). PubMed: 33986857
- Inagaki Y et al. Gan-Lu-Yin (Kanroin), Traditional Chinese Herbal Extracts, Reduces Osteoclast Differentiation In Vitro and Prevents Alveolar Bone Resorption in Rat Experimental Periodontitis J Clin Med 10:N/A (2021). PubMed: 33498415
- Zhou Q et al. Phenformin Promotes Keratinocyte Differentiation via the Calcineurin/NFAT Pathway. J Invest Dermatol 141:152-163 (2021). PubMed: 32619504
