Application
Western blot
Sample
Human Cell lysate - whole cell (Arterial endothelial cells)
Loading amount
20 µg
Specification
Arterial endothelial cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/400
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS 0,1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 120, 170 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Elena Astanina
Verified customer
Submitted May 22 2008