Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (from heart in ex-vivo culture)
Permeabilization
Yes - Triton X 100 -0,5 %
Specification
from heart in ex-vivo culture
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA 2%
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: thermofisher
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Abcam response
Thank you for submitting this AbReview about the use of ab25916 in zebrafish, a species that has not been tested before.
The homology of the immunogen with NFAT2 from zebrafish is 63%. We normally recommend a homology of 85% or higher to expect a cross-reactivity. We therefore recommend to include further controls to verify that the antibody does detect NFAT2 in zebrafish.
PubMed ID
https://www.ncbi.nlm.nih.gov/pubmed/29807022: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. N Romano
Verified customer
Submitted Aug 01 2018