Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Vector BA-1000
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Abcam response
We would like to thank the reviewer for submitting this AbReview. As the product had not yet been tested in this application we were unsure about the result. We appreciate the time and effort that the reviewer has put in to testing this product. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Hannes Kaddatz
Verified customer
Submitted Dec 31 2019