Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10)
Loading amount
20 µg
Treatment
TAA
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%Millk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-rabbit, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 10 second(s)
Bands
Specific: 95-100 kDa Non-specific: 72 kDa
Additional data
Additional Notes
Lane 1 = control, untreated
Lane 2 = vehicle, untreated
Lane 3 = 2 mM TAA, treated
Lane 4 = 4 mM TAA, treated
Gel displays MWM 95 kDa to 130 kDa
Abcam response
Sample is normal hepatocyte cell line AML12 cell, detecting NFATc1 in the absence and presence of different concentrations of TAA.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Aug 28 2020