Recombinant
RabMAb

Recombinant Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023)

Overview

  • Product name

    Anti-NFAT2 (phospho S237) antibody [EPR2377(N)]
    See all NFAT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2377(N)] to NFAT2 (phospho S237)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NFAT2 aa 200-300 (phospho S237). The exact sequence is proprietary.
    Database link: O95644

  • Positive control

    • 293 cell lysates untreated and treated with Ionomycin and TPA
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 101 kDa).

Target

  • Function

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
  • Tissue specificity

    Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.
  • Domain

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
    The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
    Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.
  • Post-translational
    modifications

    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links

  • Alternative names

    • cytoplasmic 1 antibody
    • MGC138448 antibody
    • NF ATc antibody
    • NF ATc1 antibody
    • NF-ATc antibody
    • NF-ATc1 antibody
    • NF-ATc1.2 antibody
    • NFAC1_HUMAN antibody
    • NFAT 2 antibody
    • NFAT transcription complex cytosolic component antibody
    • NFATC 1 antibody
    • NFATc antibody
    • NFATc1 antibody
    • Nuclear factor of activated T cells cytoplasmic 1 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody
    • Nuclear factor of activated T cells cytosolic component 1 antibody
    • nuclear factor of activated T-cells 'c' antibody
    • Nuclear factor of activated T-cells antibody
    see all

Images

  • Flow cytometry analysis of permealized 293 cells labeling NFAT2 (phospho S237) using ab183023 at 1/200 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green).

  • All lanes : Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023) at 1/10000 dilution

    Lane 1 : 293 lysate treated with Ionomycin and TPA
    Lane 2 : 293 lysate treated with Ionomycin and TPA, then treated with alkaline phosphatase
    Lane 3 : 293 lysate untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 101 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (breast)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
breast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 27 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up