Western blot abreview for Anti-NFAT2 (phospho S237) antibody [EPR2377(N)]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (breast)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
breast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk with 0.2% sodium azide
Dilution
1/300

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
4 minute(s) and 16 second(s)
Bands
Specific: 101 kDa

Additional data

Additional Notes
use phoso-tag gel to isolate phosphorylated and non-phosphorylated protein.

Abcam user community

Verified customer

Submitted Jun 27 2017

Sign up