Overview

  • Product name

    Anti-NFAT4/NF-ATc3 antibody [3A12]
    See all NFAT4/NF-ATc3 primary antibodies
  • Description

    Mouse monoclonal [3A12] to NFAT4/NF-ATc3
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human NFAT4/NF-ATc3 aa 70-150. (NP_775188).
    Sequence:

    HSSVLSPSFQL QSHKNYEGTC EIPESKYSPL GGPKPFECPS IQITSISPNC HQELDAHEDD LQINDPEREF LERPSRDHLY


    Database link: Q12968

  • Positive control

    • Human thymus tissue; HeLa cells.
  • General notes

    Previously labelled as NFAT4.

Properties

Applications

Our Abpromise guarantee covers the use of ab219063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1.5 - 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

Target

  • Function

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
  • Tissue specificity

    Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.
  • Domain

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications

    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links

  • Alternative names

    • C80703 antibody
    • cytoplasmic 3 antibody
    • D8Ertd281e antibody
    • NF AT4 antibody
    • NF ATc3 antibody
    • NF-AT4 antibody
    • NF-ATc3 antibody
    • NFAC3_HUMAN antibody
    • NFAT4 antibody
    • NFATc3 antibody
    • NFATx antibody
    • Nuclear factor of activated T cells, cytoplasmic 3 antibody
    • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 antibody
    • Nuclear factor of activated T-cells antibody
    • T cell transcription factor NFAT4 antibody
    • T-cell transcription factor NFAT4 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human thymus tissue labeling NFAT4 / NF-ATc3 with ab219063 at 5 µg/ml.

  • Immunofluorescent analysis of HeLa cells labeling NFAT4 / NF-ATc3 with ab219063 at 10 µg/ml.

References

ab219063 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219063.
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