Recombinant Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)
![Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)](https://www.abcam.com/ps/products/283/ab283690/Images/ab283690-472137-anti-nfat4nfatc3-antibody-epr2499468-western-blot-embryonic.jpg "Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24994-68] to NFAT4/NF-ATc3
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-NFAT4/NF-ATc3 antibody [EPR24994-68]
See all NFAT4/NF-ATc3 primary antibodies -
Description
Rabbit monoclonal [EPR24994-68] to NFAT4/NF-ATc3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Jurkat, HeLa and HEK293T whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24994-68 -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
KO cell lines
-
KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 116 kDa.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 116 kDa. |
Target
-
Function
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. -
Tissue specificity
Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle. -
Sequence similarities
Contains 1 RHD (Rel-like) domain. -
Domain
Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors. -
Post-translational
modificationsPhosphorylated by NFATC-kinase; dephosphorylated by calcineurin. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. - Information by UniProt
-
Database links
- Entrez Gene: 4775 Human
- Omim: 602698 Human
- SwissProt: Q12968 Human
- Unigene: 436585 Human
-
Alternative names
- C80703 antibody
- cytoplasmic 3 antibody
- D8Ertd281e antibody
see all
Images
-
Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)All lanes : Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690) at 1/1000 dilution
Lane 1 : Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : NFATC3 (NFAT4) knockout HEK293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution
Predicted band size: 116 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 3% NFDM/TBST
Lanes 1- 2: Merged signal (red and green). Green - ab283690 observed at 140 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab283690 was shown to react with NFATC3 (NFAT4) in wild-type HEK293T cells in western blot. Loss of signal was observed when NFATC3 (NFAT4) knockout cell line ab267300 (knockout cell lysate ab258073) was used. Wild-type HEK293T and NFATC3 (NFAT4) knockout HEK293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab283690 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Lysates were made freshly and used in WB immediately to minimize protein degradation.
-
![Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)](https://www.abcam.com/ps/products/283/ab283690/Images/ab283690-472138-anti-nfat4nfatc3-antibody-epr2499468-western-blot-cell.jpg)
Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)All lanes : Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : HEK293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 116 kDa
Observed band size: 140-160 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 3% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 31988379).
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure time: 3 minutes
-
![Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)](https://www.abcam.com/ps/products/283/ab283690/Images/ab283690-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab283690 has not yet been referenced specifically in any publications.