Key features and details
- Rabbit polyclonal to NFAT4/NF-ATc3 (phospho S165)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NFAT4/NF-ATc3 (phospho S165) antibody
See all NFAT4/NF-ATc3 primary antibodies
DescriptionRabbit polyclonal to NFAT4/NF-ATc3 (phospho S165)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human NFAT4/NF-ATc3 (phospho S165) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of Serine 165 (S-P-S(p)-P-A) derived from Human NFAT4. NCBI Protein#: NP_004546.1.
Database link: Q12968
- HeLa cell extract.
Previously labelled as NFAT4.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab182649 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab182649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 115 kDa.|
FunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
Tissue specificityIsoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
Sequence similaritiesContains 1 RHD (Rel-like) domain.
DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
modificationsPhosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
- Information by UniProt
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ab182649 has been referenced in 1 publication.
- Lapointe F et al. RPTPe promotes M2-polarized macrophage migration through ROCK2 signaling and podosome formation. J Cell Sci 133:N/A (2020). PubMed: 31722979