Overview

  • Product name

    Anti-NFAT4/NF-ATc3 (phospho S165) antibody
    See all NFAT4/NF-ATc3 primary antibodies
  • Description

    Rabbit polyclonal to NFAT4/NF-ATc3 (phospho S165)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human NFAT4/NF-ATc3 (phospho S165) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of Serine 165 (S-P-S(p)-P-A) derived from Human NFAT4. NCBI Protein#: NP_004546.1.
    Database link: Q12968

  • Positive control

    • HeLa cell extract.
  • General notes

    Previously labelled as NFAT4.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab182649 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 115 kDa.

Target

  • Function

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
  • Tissue specificity

    Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.
  • Domain

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications

    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links

  • Alternative names

    • C80703 antibody
    • cytoplasmic 3 antibody
    • D8Ertd281e antibody
    • NF AT4 antibody
    • NF ATc3 antibody
    • NF-AT4 antibody
    • NF-ATc3 antibody
    • NFAC3_HUMAN antibody
    • NFAT4 antibody
    • NFATc3 antibody
    • NFATx antibody
    • Nuclear factor of activated T cells, cytoplasmic 3 antibody
    • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 antibody
    • Nuclear factor of activated T-cells antibody
    • T cell transcription factor NFAT4 antibody
    • T-cell transcription factor NFAT4 antibody
    see all

Images

  • All lanes : Anti-NFAT4/NF-ATc3 (phospho S165) antibody (ab182649) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with antigen-specific peptide

    Predicted band size: 115 kDa

References

ab182649 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab182649.
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