Key features and details
- Rabbit polyclonal to NFAT4/NF-ATc3 (phospho S165)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NFAT4/NF-ATc3 (phospho S165) antibody
See all NFAT4/NF-ATc3 primary antibodies
DescriptionRabbit polyclonal to NFAT4/NF-ATc3 (phospho S165)
Detects endogenous levels of NFAT4/NF-ATc3 only when phosphorylated at serine 165.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human NFAT4/NF-ATc3. (Around the phosphoryation site of serine 165 (SPSPPA))
Previously labelled as NFAT4.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab59204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).|
FunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
Tissue specificityIsoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
Sequence similaritiesContains 1 RHD (Rel-like) domain.
DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
modificationsPhosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
- Information by UniProt
- C80703 antibody
- cytoplasmic 3 antibody
- D8Ertd281e antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab59204 has been referenced in 2 publications.
- Saygili E et al. Rate and irregularity of electrical activation during atrial fibrillation affect myocardial NGF expression via different signalling routes. Cell Signal : (2011). WB, ICC/IF ; Rat . PubMed: 21889978
- Lin Z et al. miR-23a functions downstream of NFATc3 to regulate cardiac hypertrophy. Proc Natl Acad Sci U S A 106:12103-8 (2009). WB ; Rat . PubMed: 19574461