Anti-NFAT4/NF-ATc3 (phospho S165) antibody (ab59204)
Key features and details
- Rabbit polyclonal to NFAT4/NF-ATc3 (phospho S165)
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NFAT4/NF-ATc3 (phospho S165) antibody
See all NFAT4/NF-ATc3 primary antibodies -
Description
Rabbit polyclonal to NFAT4/NF-ATc3 (phospho S165) -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human NFAT4/NF-ATc3 aa 100-200 (phospho S165). (Around the phosphoryation site of serine 165 (SPSPPA))
Database link: Q12968 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 21889978
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
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Notes |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 21889978 |
WB
1/500 - 1/1000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa). |
Target
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Function
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. -
Tissue specificity
Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle. -
Sequence similarities
Contains 1 RHD (Rel-like) domain. -
Domain
Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors. -
Post-translational
modificationsPhosphorylated by NFATC-kinase; dephosphorylated by calcineurin. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. - Information by UniProt
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Database links
- Entrez Gene: 4775 Human
- Entrez Gene: 18021 Mouse
- Entrez Gene: 361400 Rat
- Omim: 602698 Human
- SwissProt: Q12968 Human
- SwissProt: P97305 Mouse
- Unigene: 436585 Human
- Unigene: 383185 Mouse
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Alternative names
- C80703 antibody
- cytoplasmic 3 antibody
- D8Ertd281e antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab59204 has been referenced in 3 publications.
- Yilmaz DE et al. Immunosuppressive calcineurin inhibitor cyclosporine A induces proapoptotic endoplasmic reticulum stress in renal tubular cells. J Biol Chem 298:101589 (2022). PubMed: 35033536
- Saygili E et al. Rate and irregularity of electrical activation during atrial fibrillation affect myocardial NGF expression via different signalling routes. Cell Signal : (2011). WB, ICC/IF ; Rat . PubMed: 21889978
- Lin Z et al. miR-23a functions downstream of NFATc3 to regulate cardiac hypertrophy. Proc Natl Acad Sci U S A 106:12103-8 (2009). WB ; Rat . PubMed: 19574461