Overview

  • Product name

  • Description

    Rabbit polyclonal to NFAT5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NFAT5 aa 1481-1531. The exact sequence is proprietary.
    Database link: O94916

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000. Predicted molecular weight: 166 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.
  • Tissue specificity

    Highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon.
  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glutamine rich protein H65 antibody
    • KIAA0827 antibody
    • NF AT5 antibody
    • NF-AT5 antibody
    • NFAT 5 antibody
    • NFAT L1 antibody
    • NFAT like protein 1 antibody
    • NFAT5 antibody
    • NFAT5_HUMAN antibody
    • NFATL 1 antibody
    • NFATL1 antibody
    • NFATZ antibody
    • Nuclear factor of activated T cells 5 antibody
    • Nuclear factor of activated T cells 5 tonicity responsive antibody
    • Nuclear factor of activated T cells antibody
    • Nuclear factor of activated T-cells 5 antibody
    • OREBP antibody
    • Osmotic response element binding protein antibody
    • T cell transcription factor NFAT 5 antibody
    • T cell transcription factor NFAT5 antibody
    • T-cell transcription factor NFAT5 antibody
    • TonE binding protein antibody
    • TonE-binding protein antibody
    • TonEBP antibody
    • Tonicity responsive enhancer binding protein antibody
    • Tonicity-responsive enhancer-binding protein antibody
    see all

Images

  • All lanes : Anti-NFAT5 antibody (ab226308) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 166 kDa


    Exposure time: 30 seconds


    Cell lysates were prepared using NETN lysis buffer.

  • NFAT5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226308 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226308 at 0.4 µg/ml.

    Lane 1: ab226308 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab226308 has not yet been referenced specifically in any publications.

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