Key features and details
- Rabbit polyclonal to NFAT5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NFAT5 antibody
See all NFAT5 primary antibodies
DescriptionRabbit polyclonal to NFAT5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NFAT5 aa 1481-1531. The exact sequence is proprietary.
Database link: O94916
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226308 was affinity purified using an epitope specific to NFAT5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000. Predicted molecular weight: 166 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.
Tissue specificityHighest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon.
Sequence similaritiesContains 1 RHD (Rel-like) domain.
- Information by UniProt
- Glutamine rich protein H65 antibody
- KIAA0827 antibody
- NF AT5 antibody
All lanes : Anti-NFAT5 antibody (ab226308) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 166 kDa
Exposure time: 30 seconds
Cell lysates were prepared using NETN lysis buffer.
NFAT5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226308 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226308 at 0.4 µg/ml.
Lane 1: ab226308 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226308 has been referenced in 1 publication.
- Jiang Y et al. Transcription factor NFAT5 contributes to the glycolytic phenotype rewiring and pancreatic cancer progression via transcription of PGK1. Cell Death Dis 10:948 (2019). PubMed: 31827081