Anti-NFAT5 antibody (ab226308)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-NFAT5 antibody
See all NFAT5 primary antibodies -
Description
Rabbit polyclonal to NFAT5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NFAT5 aa 1481-1531. The exact sequence is proprietary.
Database link: O94916 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226308 was affinity purified using an epitope specific to NFAT5 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab226308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/25000. Predicted molecular weight: 166 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
-
Function
Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes. -
Tissue specificity
Highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. -
Sequence similarities
Contains 1 RHD (Rel-like) domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 10725 Human
- Omim: 604708 Human
- SwissProt: O94916 Human
- Unigene: 371987 Human
-
Alternative names
- Glutamine rich protein H65 antibody
- KIAA0827 antibody
- NF AT5 antibody
see all
Images
-
Western blot - Anti-NFAT5 antibody (ab226308)All lanes : Anti-NFAT5 antibody (ab226308) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 166 kDa
Exposure time: 30 secondsCell lysates were prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-NFAT5 antibody (ab226308)NFAT5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226308 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226308 at 0.4 µg/ml.
Lane 1: ab226308 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
ab226308 has not yet been referenced specifically in any publications.
