Key features and details
- Rabbit polyclonal to NFAT5
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NFAT5 antibody
See all NFAT5 primary antibodies
DescriptionRabbit polyclonal to NFAT5
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human NFAT5 aa 1325-1375. The exact sequence is proprietary.
Database link: O94916
- IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245452 was affinity purified using an epitope specific to NFAT5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245452 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.
Tissue specificityHighest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon.
Sequence similaritiesContains 1 RHD (Rel-like) domain.
- Information by UniProt
- Glutamine rich protein H65 antibody
- KIAA0827 antibody
- NF AT5 antibody
NFAT5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) with ab245452 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab245452 at 1 µg/ml.
Lane 1: ab245452 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
ab245452 has not yet been referenced specifically in any publications.