Product nameNFAT5 overexpression 293T lysate (whole cell)
DescriptionNFAT5 overexpression lysate
ab94270 is a 293T cell transfected lysate in which Human NFAT5 has been transiently over-expressed using a pCMV-NFAT5 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes. Similarity: Contains 1 RHD (Rel-like) domain. Tissue specificity: Highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon.
Our Abpromise guarantee covers the use of ab94270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94270 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-NFAT5 antibody - ChIP Grade (ab56997) at 1/500 dilution
Lane 1 :
NFAT5 overexpression 293T lysate (whole cell) (ab94270)
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94270 has not yet been referenced specifically in any publications.